Scholarlink

<p>Infections caused by anaerobic bacteria are common in oxygen-poor regions. Their slow metabolism makes most antibiotics less effective. And the small-molecule nutrients produced by anaerobic fermentation create a favorable growth environment for other bacteria, leading to multi-species infections. Due to the low tolerance of anaerobic bacteria to reactive oxygen species (ROS), stimulation of endogenous ROS generation could be a promising way to kill anaerobic bacteria. In this work, we used an antimicrobial peptide (AMP) (sequence: WRKFRRFKFRW-NH<sub>2</sub>, WRK for short) that induces anaerobic bacteria to produce intracellular ROS to treat infections caused by <i>Propionibacterium acnes</i> (<i>P. acnes</i>), a typical anaerobic bacterium that is often commensal in the skin. This antibacterial mechanism avoids the development of drug resistance and does not rely on oxygen. WRK peptide demonstrated excellent antimicrobial performance against <i>P. acnes</i>, inhibiting free-floating bacteria at 4 µg mL<sup>−1</sup> and destroying biofilms at 64 µg mL<sup>−1</sup>. AMP can be delivered to the dermis through microneedles and effectively inhibits the proliferation of <i>P. acnes</i> in the infected area. In a mouse model of back acne, we confirmed the therapeutic effect of this AMP microneedle on <i>P. acnes</i> infection <i>in vivo</i>. The antibacterial microneedle treatment significantly reduced inflammation, demonstrating superior therapeutic efficacy compared to commercial clindamycin gel.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Antimicrobial peptide microneedles with endogenous ROS-generating capacity for the treatment of anaerobic Propionibacterium acnes infection

  • 期刊论文

  • 发表日期：

    2026年2月27日

查看全文
  • Kejia Li,
  • Aguo Cheng,
  • Xiaoyan Ju,
  • Jinzhao Ou,
  • Chenxiao Wan,
  • Meng Zhu,
  • Yunhua Gao,
  • Ye Tian,
  • Zhongwei Niu

摘要

Infections caused by anaerobic bacteria are common in oxygen-poor regions. Their slow metabolism makes most antibiotics less effective. And the small-molecule nutrients produced by anaerobic fermentation create a favorable growth environment for other bacteria, leading to multi-species infections. Due to the low tolerance of anaerobic bacteria to reactive oxygen species (ROS), stimulation of endogenous ROS generation could be a promising way to kill anaerobic bacteria. In this work, we used an antimicrobial peptide (AMP) (sequence: WRKFRRFKFRW-NH2, WRK for short) that induces anaerobic bacteria to produce intracellular ROS to treat infections caused by Propionibacterium acnes (P. acnes), a typical anaerobic bacterium that is often commensal in the skin. This antibacterial mechanism avoids the development of drug resistance and does not rely on oxygen. WRK peptide demonstrated excellent antimicrobial performance against P. acnes, inhibiting free-floating bacteria at 4 µg mL−1 and destroying biofilms at 64 µg mL−1. AMP can be delivered to the dermis through microneedles and effectively inhibits the proliferation of P. acnes in the infected area. In a mouse model of back acne, we confirmed the therapeutic effect of this AMP microneedle on P. acnes infection in vivo. The antibacterial microneedle treatment significantly reduced inflammation, demonstrating superior therapeutic efficacy compared to commercial clindamycin gel.