In this study we consider a Sturm-Liouville equation \(\begin{aligned} (k(x)y')'+ \sigma (x)y=0, \end{aligned}\) defined on two disjoint intervals [a, c) and (c, b], the left and right parts of the solutions of which are connected with interaction conditions of the form \( y(c+0)=\alpha y(c-0), y'(c+0)=\beta y'(c-0),\) the so-called transmission conditions. We have obtained new Picone type identities to establish some comparison and oscillation theorems. In the special case \(\alpha =\beta =1,\) our results are equivalent to the corresponding classical results, so the results obtained extend and generalize the corresponding results from the Sturm’s classical comparison and oscillation theory.