Scholarlink

<p>Dental caries, a highly prevalent oral disease, is primarily driven by pathogenic biofilms; however, current antimicrobials exhibit limited efficacy and poor specificity against cariogenic biofilms. Although nanobiocatalysts that can produce reactive oxygen species represent a promising alternative to conventional antimicrobials, most current designs fail to achieve robust bacterial interaction and exhibit insufficient disruption of biofilm integrity. To address these challenges, we report the de novo design of phage-inspired artificial peroxidases (IrNC@TiO<sub>2</sub>) featuring a robust sub-nanometer cluster site and urchin-like topography, which enables efficient oral biofilm elimination and dental caries prevention. Structural characterization confirmed that sub-nanometer Ir clusters are stably anchored to the TiO<sub>2</sub> support via Ir–O coordination. Leveraging the robust enzymatic activity of Ir clusterzymes and the topological advantages of the spiky substrate, IrNC@TiO<sub>2</sub> exhibits potent multi-enzyme mimetic activity, generating substantial amounts of ·O<sub>2</sub><sup>−</sup> and HClO to effectively capture and eradicate planktonic <i>Streptococcus mutans</i> and suppress biofilm formation. In a caries model, IrNC@TiO<sub>2</sub> significantly inhibited tooth surface biofilm development, prevented enamel demineralization, and reduced caries incidence. The material also demonstrated negligible cytotoxicity and outperformed conventional non-abrasive additives in tooth-whitening assays. This work introduces a robust and efficient ROS-generating platform for oral health care and proposes a promising solution for clinical caries prevention.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Phage-Inspired Artificial Peroxidases with Robust Sub–Nanometer Cluster Sites for Efficient Oral Biofilm Elimination and Dental Caries Prevention

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月26日

查看全文
  • Huang Zhu,
  • Ting Wang,
  • Shihuan Gao,
  • Wei Geng,
  • Tian Ma,
  • Liang Cheng,
  • Xianglong Han,
  • Jiuhong Deng,
  • Shanshan Gao,
  • Chong Cheng

摘要

Dental caries, a highly prevalent oral disease, is primarily driven by pathogenic biofilms; however, current antimicrobials exhibit limited efficacy and poor specificity against cariogenic biofilms. Although nanobiocatalysts that can produce reactive oxygen species represent a promising alternative to conventional antimicrobials, most current designs fail to achieve robust bacterial interaction and exhibit insufficient disruption of biofilm integrity. To address these challenges, we report the de novo design of phage-inspired artificial peroxidases (IrNC@TiO2) featuring a robust sub-nanometer cluster site and urchin-like topography, which enables efficient oral biofilm elimination and dental caries prevention. Structural characterization confirmed that sub-nanometer Ir clusters are stably anchored to the TiO2 support via Ir–O coordination. Leveraging the robust enzymatic activity of Ir clusterzymes and the topological advantages of the spiky substrate, IrNC@TiO2 exhibits potent multi-enzyme mimetic activity, generating substantial amounts of ·O2− and HClO to effectively capture and eradicate planktonic Streptococcus mutans and suppress biofilm formation. In a caries model, IrNC@TiO2 significantly inhibited tooth surface biofilm development, prevented enamel demineralization, and reduced caries incidence. The material also demonstrated negligible cytotoxicity and outperformed conventional non-abrasive additives in tooth-whitening assays. This work introduces a robust and efficient ROS-generating platform for oral health care and proposes a promising solution for clinical caries prevention.