<p>Adverse childhood experiences (ACE) are associated with negative long-term health consequences, such as autoimmunity. How characteristics of exposure, including types and timing, relate to autoimmunity remains less understood. The objective of the current study is to examine how characteristics of ACE exposure are related to autoimmunity and health-related quality of life (HrQoL). Canadian undergraduate psychology students (<i>N</i> = 643) ages 17 – 40 participated in an online survey. The survey collected on 10-types of adversity, 83 autoimmune symptoms and diseases, and 8 domains of HRQoL, which were analyzed using linear modelling. Participants with clinical ACE exposure reported more autoimmune conditions on average compared to those with nonclinical exposure (<i>β</i> = .33, <i>p</i> &lt; .001). As the severity of the exposure increased, so did the number of reported autoimmune conditions (<i>β</i> = .47, <i>p</i> &lt; .001). The number of different types of exposure was also associated with an increase in autoimmune conditions (<i>β</i> = .37, <i>p</i> &lt; .001), especially among those who had multiple types of exposure. The timing of exposure was related to more autoimmune conditions (<i>β</i> = .26, <i>p</i> &lt; .001), particularly for those with early exposure (ages 0—5) compared to late exposure (ages 9—16, <i>p</i> &lt; .01). Reduced HRQoL was also observed across characteristics of clinical exposure (<i>β</i> range = -.46—-.22, <i>p</i> &lt; .001). Exposure to childhood adversity was significantly associated with more autoimmune conditions in adulthood, poor HRQoL, and varied across characteristics of exposure.</p>