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<p>Auxin biosynthesis and transport are critical processes for <i>in vitro</i> tissue culture development, but the regulatory responses associated with these processes are still poorly understood in plants. We investigated the role of auxin transport during somatic embryo differentiation in sugarcane callus using an auxin transport inhibitor through a high-throughput proteomic approach. Four concentrations of 2,3,5-triiodobenzoic acid (TIBA 0, 1, 10, and 100&#xa0;µM) were tested on sugarcane embryogenic callus during the pre-maturation phase. Treatment with 100&#xa0;µM TIBA inhibited the differentiation of embryogenic callus into somatic embryos. Then, embryogenic callus from control and 100&#xa0;µM TIBA treatments were compared via proteomic analysis. A total of 1221 proteins were found, of which 740 were differentially accumulated (DAP) in the 100&#xa0;µM TIBA/Control comparison. Among the DAPs, 33 were unique to the control treatment, 31 were unique to the 100&#xa0;µM TIBA pre-maturation treatment, and 415 down-regulated and 261 up-regulated. Inhibition of auxin transport impaired the accumulation and predicted protein interactions involved in embryonic development, ubiquitination, and energy metabolism. The concentrations of spermidine synthase and spermidine were reduced, suggesting an association between auxin and polyamines during development. The down-regulation of the proteins chaperone BOBBER1, Cullin-associated NEDD8-dissociated protein 1, and Receptor-like kinase TMK2 could be associated with auxin responses and the predicted interactions between proteasome members found during the differentiation of somatic embryos. These new findings demonstrate the role of auxin transport in downstream responses during somatic embryo differentiation and open new avenues for exploring the potential of sugarcane for biotechnological purposes.</p> Graphical abstract <p></p>

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Disruption of auxin transport impairs somatic embryo development and proteome dynamics in sugarcane

  • 期刊论文

  • 发表日期：

    2025年7月1日

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  • Lucas Rodrigues Xavier,
  • Daniel Dastan Rezabala Pacheco,
  • Laís dos Santos Conceição,
  • Caio Cezar Guedes Corrêa,
  • Roberta Pena da Paschoa,
  • Lucas Gomes do Espirito Santo,
  • Felipe Astolpho Almeida,
  • Vitor Batista Pinto,
  • Tadeu dos Reis de Oliveira,
  • Claudete Santa-Catarina,
  • Vanildo Silveira

摘要

Auxin biosynthesis and transport are critical processes for in vitro tissue culture development, but the regulatory responses associated with these processes are still poorly understood in plants. We investigated the role of auxin transport during somatic embryo differentiation in sugarcane callus using an auxin transport inhibitor through a high-throughput proteomic approach. Four concentrations of 2,3,5-triiodobenzoic acid (TIBA 0, 1, 10, and 100 µM) were tested on sugarcane embryogenic callus during the pre-maturation phase. Treatment with 100 µM TIBA inhibited the differentiation of embryogenic callus into somatic embryos. Then, embryogenic callus from control and 100 µM TIBA treatments were compared via proteomic analysis. A total of 1221 proteins were found, of which 740 were differentially accumulated (DAP) in the 100 µM TIBA/Control comparison. Among the DAPs, 33 were unique to the control treatment, 31 were unique to the 100 µM TIBA pre-maturation treatment, and 415 down-regulated and 261 up-regulated. Inhibition of auxin transport impaired the accumulation and predicted protein interactions involved in embryonic development, ubiquitination, and energy metabolism. The concentrations of spermidine synthase and spermidine were reduced, suggesting an association between auxin and polyamines during development. The down-regulation of the proteins chaperone BOBBER1, Cullin-associated NEDD8-dissociated protein 1, and Receptor-like kinase TMK2 could be associated with auxin responses and the predicted interactions between proteasome members found during the differentiation of somatic embryos. These new findings demonstrate the role of auxin transport in downstream responses during somatic embryo differentiation and open new avenues for exploring the potential of sugarcane for biotechnological purposes.

Graphical abstract