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<p>Carrot suspension-cultured cells (SCC) were treated with different inhibitors to analyze the involvement of calcium (Ca<sup>2+</sup>), nitric oxide (NO), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and phosphorylation/dephosphorylation events in the phytosterol production triggered by 50&#xa0;mM methyl-β-cyclodextrins (M-β-CD). The results demonstrated that the accumulation of phytosterols triggered by M-β-CD depends on an uptake of Ca<sup>2+</sup> from the extracellular medium. Similarly, protein phosphorylation/dephosphorylation events are involved in the signal transduction pathways mediated by M-β-CD as the phytosterol production is dependent on both the activity of tyrosine phosphatases and the phosphorylation status of serin/threonine protein kinases. Finally, the results indicated that NO also partially contributed to extracellular accumulation of phytosterols triggered by M-β-CD in carrot plant suspension-cultured cells. However, no change in the extracellular accumulation of phytosterols was observed in the presence of H<sub>2</sub>O<sub>2</sub> inhibitor, suggesting that H<sub>2</sub>O<sub>2</sub> does not play a key role in the signaling pathway triggered by M-β-CD for the extracellular accumulation of these metabolites.</p>

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Calcium and nitric oxide modulate phytosterol production in cyclodextrins-elicited carrot cells

  • 期刊论文

  • 发表日期：

    2025年4月2日

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  • Ana Belén Sabater-Jara,
  • M. A. Pedreño,
  • Lorena Almagro

摘要

Carrot suspension-cultured cells (SCC) were treated with different inhibitors to analyze the involvement of calcium (Ca2+), nitric oxide (NO), hydrogen peroxide (H2O2) and phosphorylation/dephosphorylation events in the phytosterol production triggered by 50 mM methyl-β-cyclodextrins (M-β-CD). The results demonstrated that the accumulation of phytosterols triggered by M-β-CD depends on an uptake of Ca2+ from the extracellular medium. Similarly, protein phosphorylation/dephosphorylation events are involved in the signal transduction pathways mediated by M-β-CD as the phytosterol production is dependent on both the activity of tyrosine phosphatases and the phosphorylation status of serin/threonine protein kinases. Finally, the results indicated that NO also partially contributed to extracellular accumulation of phytosterols triggered by M-β-CD in carrot plant suspension-cultured cells. However, no change in the extracellular accumulation of phytosterols was observed in the presence of H2O2 inhibitor, suggesting that H2O2 does not play a key role in the signaling pathway triggered by M-β-CD for the extracellular accumulation of these metabolites.