Purpose of Review <p>The purpose of this review is to highlight the emerging role of lipid metabolism in shaping antifungal resistance and pathogenesis in yeast, with a particular focus on the recent studies on pathogenic species of <i>Candida</i>. While genetic and protein-based mechanisms of resistance have been widely studied, the contribution of lipids, especially sphingolipids, sterols, and phosphoglycerides to drug tolerance, membrane remodelling, and virulence remains underappreciated.</p> Recent Findings <p>Among the various cellular components influencing antifungal resistance and virulence, lipids have emerged as key players, influencing fungal pathogenesis, maintaining membrane integrity, mediating drug resistance, and modulating interactions with the host. A growing body of research has uncovered multiple layers at which lipid metabolism intersects with fungal adaptation, highlighting its centrality in both survival and pathogenicity.</p> Summary <p>This review, though not exhaustive, aims to highlight the expanding role of lipids in antifungal resistance and pathogenesis in pathogenic yeasts.</p>