In the current study, an environmentally friendly process using pulsed laser ablation in a naturally acidic medium, such as tomato juice extract, was employed to synthesize Al2O3 nanoparticles and demonstrate their potential for antibacterial applications. Nd: YAG laser with different laser energies (300, 700) mJ was used. The nanostructure, morphology, optical properties, contact angle, and antibacterial activity of aluminum oxide nanoparticles were studied. The results of UV–vis spectroscopy of the produced NPs showed a peak absorption at (254) nm for NPs created at laser energy 300 mJ, and two peaks of absorption at (212 and 248) nm for NPs created at laser energy 700 mJ. The bandgap values of Al2O3NPs increase from 3.15 to 3.25 eV with the increase of laser energy. The dynamic light scattering (DLS) showed mean diameters of about 53 and 40 nm with zeta potential about (+ 23, + 39) mV for laser energies (300,700) mJ. The size and shape of the generated NPs were examined using field emission scanning electron microscopy (FESEM). The results showed spherical shape with small sizes ranging from 10 to 53 nm for Al2O3NPs produced at laser energy 700 mJ, and larger sizes ranged from 10 to 110 nm with spherical and irregular shapes for Al2O3NPs produced at laser energy 300 mJ. The results of Transmission Electron Microscopy (TEM) showed that the Al2O3 NPs created at lower laser energy have both agglomerated and isolated particles with irregular and spherical forms. Atomic force microscopy (AFM) was utilized to examine the morphology and surface roughness. the results showed an increase in the roughness of Al2O3 NPs with the increase of laser energy. The results of X-ray diffraction (XRD) emphasized the creation of high-purity crystalline Al2O3 NPs. The mean grain size (D) of Al2O3 NPs produced at laser energies of 300 and 700 mJ was found to be around 24 and 19 nm, respectively. The produced NPs at laser energy 300 mJ exhibited a contact angle between 72o and 73o and for the NPs produced at laser energy 700 mJ was between 68 ° and 70o, which is an excellent outcome referred to as hydrophilic NPs. The results of antibacterial activity indicate that using tomato juice extract as an acidic environment for aluminum oxide NPs exhibited high antibacterial activity against Gram-positive: Staphylococcus aureus. The preparation procedure, characterization methods, effect of laser energy, and effect of the tomato juice extract on the size, stability, and antibacterial effectivity have been discussed.

Investigate the Effect of Acidic Environment on the Characteristics and Antibacterial Activity of Al₂O₃ Nanoparticles Prepared by Laser Ablation

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发表日期：

2025年7月25日

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Ali H. Attallah,
Farah Shamil Abdulwahid,
Yasir A. Ali,
Adawiya J. Haider

摘要

In the current study, an environmentally friendly process using pulsed laser ablation in a naturally acidic medium, such as tomato juice extract, was employed to synthesize Al2O3 nanoparticles and demonstrate their potential for antibacterial applications. Nd: YAG laser with different laser energies (300, 700) mJ was used. The nanostructure, morphology, optical properties, contact angle, and antibacterial activity of aluminum oxide nanoparticles were studied. The results of UV–vis spectroscopy of the produced NPs showed a peak absorption at (254) nm for NPs created at laser energy 300 mJ, and two peaks of absorption at (212 and 248) nm for NPs created at laser energy 700 mJ. The bandgap values of Al₂O₃NPs increase from 3.15 to 3.25 eV with the increase of laser energy. The dynamic light scattering (DLS) showed mean diameters of about 53 and 40 nm with zeta potential about (+ 23, + 39) mV for laser energies (300,700) mJ. The size and shape of the generated NPs were examined using field emission scanning electron microscopy (FESEM). The results showed spherical shape with small sizes ranging from 10 to 53 nm for Al₂O₃NPs produced at laser energy 700 mJ, and larger sizes ranged from 10 to 110 nm with spherical and irregular shapes for Al₂O₃NPs produced at laser energy 300 mJ. The results of Transmission Electron Microscopy (TEM) showed that the Al₂O₃ NPs created at lower laser energy have both agglomerated and isolated particles with irregular and spherical forms. Atomic force microscopy (AFM) was utilized to examine the morphology and surface roughness. the results showed an increase in the roughness of Al₂O₃ NPs with the increase of laser energy. The results of X-ray diffraction (XRD) emphasized the creation of high-purity crystalline Al₂O₃ NPs. The mean grain size (D) of Al₂O₃ NPs produced at laser energies of 300 and 700 mJ was found to be around 24 and 19 nm, respectively. The produced NPs at laser energy 300 mJ exhibited a contact angle between 72^o and 73^o and for the NPs produced at laser energy 700 mJ was between 68 ° and 70^o, which is an excellent outcome referred to as hydrophilic NPs. The results of antibacterial activity indicate that using tomato juice extract as an acidic environment for aluminum oxide NPs exhibited high antibacterial activity against Gram-positive: Staphylococcus aureus. The preparation procedure, characterization methods, effect of laser energy, and effect of the tomato juice extract on the size, stability, and antibacterial effectivity have been discussed.

Investigate the Effect of Acidic Environment on the Characteristics and Antibacterial Activity of Al2O3 Nanoparticles Prepared by Laser Ablation

摘要

Investigate the Effect of Acidic Environment on the Characteristics and Antibacterial Activity of Al₂O₃ Nanoparticles Prepared by Laser Ablation