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<p>Daily flowering dynamics in rice is closely associated with the efficiency of hybrid seeds production. In this study, the Transgene Killer CRISPR (TKC) system was used to modify <i>EMF1</i>, a regulator of daily flowering patterns previously identified as a regulator of daily flowering patterns, in the <i>Japonica</i> rice cultivar Hanhui89. Sequencing analysis of T<sub>0</sub> transgenic plants revealed high editing efficiency of the TKC system at the target <i>EMF1</i> locus. Among the edited plants, 6.0% carried homozygous mutations, 75.8% carried heterozygous mutations, and the remaining 18.2% carried biallelic mutations. Evaluation of agronomic traits in homozygous T<sub>2</sub> mutant lines revealed that the daily flowering dynamics was advanced by approximately two hours compared to wild-type (WT) controls. Notably, thousand-grain weight was significantly increased, whereas all other agronomic traits remained unchanged. These findings suggest that <i>EMF1</i> knockout alleles generated through the TKC system, could be utilized to modify daily flowering dynamics in <i>Japonica</i> rice.</p>

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CRISPR-mediated editing of the EMF1 gene improved daily flowering dynamics in Japonica rice

  • 期刊论文

  • 发表日期：

    2026年6月15日

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  • E Li,
  • Nai Wu,
  • Mengchen Li,
  • Yanan Wang,
  • Feiming Wang,
  • Xingxing Luo,
  • Ao Li,
  • Anning Zhang

摘要

Daily flowering dynamics in rice is closely associated with the efficiency of hybrid seeds production. In this study, the Transgene Killer CRISPR (TKC) system was used to modify EMF1, a regulator of daily flowering patterns previously identified as a regulator of daily flowering patterns, in the Japonica rice cultivar Hanhui89. Sequencing analysis of T0 transgenic plants revealed high editing efficiency of the TKC system at the target EMF1 locus. Among the edited plants, 6.0% carried homozygous mutations, 75.8% carried heterozygous mutations, and the remaining 18.2% carried biallelic mutations. Evaluation of agronomic traits in homozygous T2 mutant lines revealed that the daily flowering dynamics was advanced by approximately two hours compared to wild-type (WT) controls. Notably, thousand-grain weight was significantly increased, whereas all other agronomic traits remained unchanged. These findings suggest that EMF1 knockout alleles generated through the TKC system, could be utilized to modify daily flowering dynamics in Japonica rice.