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<p>Linseed (<i>Linum usitatissimum</i> L.) is highly sensitive to drought stress, which results in substantial yield loss. In linseed drought tolerant accessions have been reported, however, there is limited information on the candidate genes and their allelic variation. In this study, a diverse panel of 12 accessions (seven tolerant and five susceptible accessions) was studied for genetic variation of ten potential candidate genes (<i>LEA5</i>, <i>AP2/ERF</i>, <i>WRKY3</i>, <i>MYB1</i>, <i>CKS1</i>, <i>MIZ1</i>, <i>LushsfB1a</i>, <i>Dehydrin1</i>, <i>SAUR</i>, and <i>PLD_Delta</i>). Six of the ten genes, including <i>LuSAUR</i>, <i>LuMIZ1</i>, <i>LuCKS1</i>, <i>LuPLD-Delta</i>, <i>LuWRKY3</i>, and <i>LuMYB1</i>, showed allelic variation in the studied accessions. The allelic variations included SNPs (synonymous and non-synonymous) and InDels in the genic regions. These variations defined two distinct blocks: Haplotype 1 (Hap1) and Haplotype 2 (Hap2). Hap2 was associated with superior root system architecture (RSA) plasticity, physiological homeostasis, and more robust transcriptional induction of <i>LuMIZ1</i> (4.43-fold) and <i>LuPLD</i> (3.40-fold) under drought stress. Conversely, susceptible Hap1 accessions harboured allelic variants with attenuated gene expression under drought. Homology protein modelling of variants suggested that Hap2 has relatively stable conformational properties compared to Hap1. This study unravels the novel allelic variants of key candidate genes for drought tolerance in linseed and constitutes crucial genomic resources in linseed breeding for water deficit areas.</p> Graphical Abstract <p></p>

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Allelic variation in root architecture and stress responsive genes in contrasting linseed accessions and their potential role in drought stress tolerance

  • 期刊论文

  • 发表日期：

    2026年4月25日

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  • Sheela,
  • Shashank Kumar Yadav,
  • Manoj Kumar,
  • Dhammaprakash Pandhari Wankhede,
  • Vikender Kaur

摘要

Linseed (Linum usitatissimum L.) is highly sensitive to drought stress, which results in substantial yield loss. In linseed drought tolerant accessions have been reported, however, there is limited information on the candidate genes and their allelic variation. In this study, a diverse panel of 12 accessions (seven tolerant and five susceptible accessions) was studied for genetic variation of ten potential candidate genes (LEA5, AP2/ERF, WRKY3, MYB1, CKS1, MIZ1, LushsfB1a, Dehydrin1, SAUR, and PLD_Delta). Six of the ten genes, including LuSAUR, LuMIZ1, LuCKS1, LuPLD-Delta, LuWRKY3, and LuMYB1, showed allelic variation in the studied accessions. The allelic variations included SNPs (synonymous and non-synonymous) and InDels in the genic regions. These variations defined two distinct blocks: Haplotype 1 (Hap1) and Haplotype 2 (Hap2). Hap2 was associated with superior root system architecture (RSA) plasticity, physiological homeostasis, and more robust transcriptional induction of LuMIZ1 (4.43-fold) and LuPLD (3.40-fold) under drought stress. Conversely, susceptible Hap1 accessions harboured allelic variants with attenuated gene expression under drought. Homology protein modelling of variants suggested that Hap2 has relatively stable conformational properties compared to Hap1. This study unravels the novel allelic variants of key candidate genes for drought tolerance in linseed and constitutes crucial genomic resources in linseed breeding for water deficit areas.

Graphical Abstract