Scholarlink

<p>This study employed GC–MS-based volatile metabolomics to systematically analyze and compare the metabolic profiles of root exudates from two host plants of <i>Cynomorium songaricum</i>—<i>Peganum multisectum</i> and <i>Zygophyllum xanthoxylon</i>—under hydroponic and aeroponic cultivation conditions, collected using a 24-h deionized water immersion method. A total of 1411, 1535, 1412, and 1585 metabolites were identified across the four sample groups. Multivariate statistical analyses, including principal component analysis and orthogonal partial least squares discriminant analysis, revealed that the cultivation method exerted a significantly greater influence on root exudate composition than the host species. Metabolic pathway enrichment analysis further demonstrated that these differential metabolites were primarily enriched in key metabolic pathways, including monoterpenoid biosynthesis, sesquiterpenoid and triterpenoid biosynthesis, phenylpropanoid biosynthesis, and arginine and proline metabolism. Secondary metabolites, such as terpenoids, were significantly upregulated under aeroponic conditions. Based on literature evidence, the screened differential metabolites include compounds previously reported to exhibit plant seed germination-regulating activities (e.g., limonene, which promotes seed germination at low concentrations) as well as compounds with allelopathic effects. Although the biological functions of these potential compounds still require rigorous validation through seed germination experiments, this study provides a foundational molecular library and exploratory research framework for screening candidate signaling molecules that may promote <i>C. songaricum</i> seed germination. It proposes a stress-induced cultivation strategy that offers new insights for future research on artificially regulating the rhizosphere environment to promote <i>C. songaricum</i> seed germination.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Aeroponics as a novel strategy to enrich stimulants in root exudates of Cynomorium songaricum host plant: A metabolomic perspective

  • 期刊论文

  • 发表日期：

    2026年2月5日

查看全文
  • Yu Tang,
  • Changmao Chen,
  • Yubi Zhou,
  • Jie Wang

摘要

This study employed GC–MS-based volatile metabolomics to systematically analyze and compare the metabolic profiles of root exudates from two host plants of Cynomorium songaricum—Peganum multisectum and Zygophyllum xanthoxylon—under hydroponic and aeroponic cultivation conditions, collected using a 24-h deionized water immersion method. A total of 1411, 1535, 1412, and 1585 metabolites were identified across the four sample groups. Multivariate statistical analyses, including principal component analysis and orthogonal partial least squares discriminant analysis, revealed that the cultivation method exerted a significantly greater influence on root exudate composition than the host species. Metabolic pathway enrichment analysis further demonstrated that these differential metabolites were primarily enriched in key metabolic pathways, including monoterpenoid biosynthesis, sesquiterpenoid and triterpenoid biosynthesis, phenylpropanoid biosynthesis, and arginine and proline metabolism. Secondary metabolites, such as terpenoids, were significantly upregulated under aeroponic conditions. Based on literature evidence, the screened differential metabolites include compounds previously reported to exhibit plant seed germination-regulating activities (e.g., limonene, which promotes seed germination at low concentrations) as well as compounds with allelopathic effects. Although the biological functions of these potential compounds still require rigorous validation through seed germination experiments, this study provides a foundational molecular library and exploratory research framework for screening candidate signaling molecules that may promote C. songaricum seed germination. It proposes a stress-induced cultivation strategy that offers new insights for future research on artificially regulating the rhizosphere environment to promote C. songaricum seed germination.

Graphical Abstract