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<p>The abscisic acid-, stress-, and ripening-induced (ASR) proteins play important roles in protecting plants from adverse environmental conditions. However, the biological functions of ASRs in cucumber remain poorly understood. In this study, we characterized the cucumber <i>CsASR1</i> gene and demonstrated its positive role in salt stress tolerance. CsASR1 was localized to the endoplasmic reticulum (ER) and showed no transactivation activity. Compared to controls, p<i>CsASR1</i>::GUS transgenic <i>Arabidopsis</i> seedlings exhibited stronger GUS activity under salt and H<sub>2</sub>O<sub>2</sub> treatments but significantly reduced staining following ABA treatment. Ectopic expression of <i>CsASR1</i> enhanced <i>Arabidopsis</i> salt tolerance, as evidenced by increased chlorophyll levels and decreased relative electrolyte leakage under salt stress conditions. Furthermore, <i>CsASR1</i>-overexpressing plants displayed enhanced reactive oxygen species (ROS) scavenging capability during salt stress, including elevated activities of antioxidant enzymes that led to reduced O<sub>2</sub><sup>·</sup>⁻ and H<sub>2</sub>O<sub>2</sub> accumulation. CsASR1-mediated salt tolerance was further supported by the activation of stress-responsive genes (<i>AtP5CS</i>, <i>AtRD29B</i> and <i>AtWRKY25</i>), while <i>AtNCED3</i> expression was downregulated. These findings collectively demonstrate that <i>CsASR1</i> plays crucial roles in salt stress responses by maintaining ROS homeostasis and regulating stress-related gene expression.</p>

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CsASR1 from cucumber (Cucumis sativus L.) positively regulates salt stress tolerance in Arabidopsis

  • 期刊论文

  • 发表日期：

    2026年2月5日

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  • Weifeng Huang,
  • Hao Wu,
  • Jie Zheng,
  • Haoju Liu,
  • Liuhua Guo,
  • Shiqiang Liu,
  • Yong Zhou

摘要

The abscisic acid-, stress-, and ripening-induced (ASR) proteins play important roles in protecting plants from adverse environmental conditions. However, the biological functions of ASRs in cucumber remain poorly understood. In this study, we characterized the cucumber CsASR1 gene and demonstrated its positive role in salt stress tolerance. CsASR1 was localized to the endoplasmic reticulum (ER) and showed no transactivation activity. Compared to controls, pCsASR1::GUS transgenic Arabidopsis seedlings exhibited stronger GUS activity under salt and H2O2 treatments but significantly reduced staining following ABA treatment. Ectopic expression of CsASR1 enhanced Arabidopsis salt tolerance, as evidenced by increased chlorophyll levels and decreased relative electrolyte leakage under salt stress conditions. Furthermore, CsASR1-overexpressing plants displayed enhanced reactive oxygen species (ROS) scavenging capability during salt stress, including elevated activities of antioxidant enzymes that led to reduced O2·⁻ and H2O2 accumulation. CsASR1-mediated salt tolerance was further supported by the activation of stress-responsive genes (AtP5CS, AtRD29B and AtWRKY25), while AtNCED3 expression was downregulated. These findings collectively demonstrate that CsASR1 plays crucial roles in salt stress responses by maintaining ROS homeostasis and regulating stress-related gene expression.