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<p>Drought is the main abiotic stress that impacts biomass productivity and grain yield in crops. Dehydrins are part of the Late Embryogenesis Abundant protein family. They are typically upregulated under water-limited conditions, playing a key role in plant response and adaptation to stressful environments. However, the specific function of dehydrins and their relationship with plant functional traits and drought-related physiology remain poorly understood. <i>Brachypodium distachyon</i> L. is a well-studied monocot model plant for temperate grasses, yet DHN proteins in <i>Brachypodium</i> have not been extensively researched. To our knowledge, this is the first functional validation in <i>Brachypodium</i> of a dehydrin gene (BdDHN3) demonstrating a causal improvement in drought tolerance, together with a comparative analysis of BdDHN3 expression across 11 genetically distinct lines. Thus, we first examined variations in the expression level of the BdDHN3 gene among 11 genetically distinct <i>B. distachyon</i> inbred lines to assess the genetic consistency of gene expression. Second, we generated transgenic plants with overexpression constructs in the Bd21 line to analyze drought response at both the functional and genetic expression levels. On average, BdDHN3 expression was 447.2 times higher under drought conditions compared to the control across <i>B. distachyon</i> lines. Overexpression of BdDHN3 resulted in enhanced drought tolerance, as plants overexpressing BdDHN3 retained more water, exhibited lower cell damage, and had higher proline contents under water stress conditions compared to wild-type (WT) plants. These findings suggest that BdDHN3 may play an important role in the plant response to drought stress. Given its proximity and gene synteny to other significant temperate cereal crops, our results indicate that DHN3 proteins may also be relevant for temperate grasses with socio-economic importance.</p>

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Over-expression of the Brachypodium dehydrin gene, BdDHN3, enhances drought tolerance in Brachypodium distachyon (Poaceae)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月24日

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  • Luisa María Martínez,
  • Ana María Fernández-Ocaña,
  • Pedro J. Rey,
  • Antonio J. Manzaneda

摘要

Drought is the main abiotic stress that impacts biomass productivity and grain yield in crops. Dehydrins are part of the Late Embryogenesis Abundant protein family. They are typically upregulated under water-limited conditions, playing a key role in plant response and adaptation to stressful environments. However, the specific function of dehydrins and their relationship with plant functional traits and drought-related physiology remain poorly understood. Brachypodium distachyon L. is a well-studied monocot model plant for temperate grasses, yet DHN proteins in Brachypodium have not been extensively researched. To our knowledge, this is the first functional validation in Brachypodium of a dehydrin gene (BdDHN3) demonstrating a causal improvement in drought tolerance, together with a comparative analysis of BdDHN3 expression across 11 genetically distinct lines. Thus, we first examined variations in the expression level of the BdDHN3 gene among 11 genetically distinct B. distachyon inbred lines to assess the genetic consistency of gene expression. Second, we generated transgenic plants with overexpression constructs in the Bd21 line to analyze drought response at both the functional and genetic expression levels. On average, BdDHN3 expression was 447.2 times higher under drought conditions compared to the control across B. distachyon lines. Overexpression of BdDHN3 resulted in enhanced drought tolerance, as plants overexpressing BdDHN3 retained more water, exhibited lower cell damage, and had higher proline contents under water stress conditions compared to wild-type (WT) plants. These findings suggest that BdDHN3 may play an important role in the plant response to drought stress. Given its proximity and gene synteny to other significant temperate cereal crops, our results indicate that DHN3 proteins may also be relevant for temperate grasses with socio-economic importance.