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<p>The present rice cultivars have reached a yield plateau primarily due to the restricted use of germplasm within <i>Oryza sativa</i> cultivars as parental lines in breeding programmes. Wild ancestors and related species comprise a vital source of genetic material to break the yield barrier. The present investigation was carried out for identification and introgression of yield enhancing QTLs by developing 190 BC<sub>2</sub>F<sub>2</sub> backcross population derived from a cross between Samba Mahsuri*2/<i>O. rufipogon</i> (IRGC106106). Polymorphic SSR markers spanning the genome were used to genotype the mapping population to construct a linkage map of 1893&#xa0;cM (Kosambi). The CIM identified a total of 47 significant QTLs at <i>P</i> &lt; 0.001 (LOD &gt; 2.50) for ten yield traits distributed across 11 chromosomes. Among these, <i>O. rufipogon</i> alleles contributed a beneficial effect on 22 QTLs. The cumulative phenotypic variance explained by donor-derived QTLs for target traits was ranging from 2.12 to 32.65% with a single major QTL <i>qPL9.1</i> with 29.14% PVE. Yield increase in introgression lines over Samba Mahsuri (BPT5204) was attributed by beneficial allele from <i>O. rufipogon</i> for the traits such as productive tillers, panicle length, test weight and grain yield per plant. Three major QTLs with more than 10% phenotypic variance observed. Significant genomic region <i>qPL9.1</i> was detected from <i>O. rufipogon</i> associated with panicle length, with 29.14% of observed phenotypic variance. <i>qDF1.1</i> and <i>qPH2.1</i> are the other major QTLs from recurrent parent Samba Mahsuri with 29.33% and 29.20% phenotypic variance respectively. There were six QTL hotspots viz., <i>qY1a</i>, <i>qY1b</i>, <i>qY2</i>, <i>qY3</i>, <i>qY7</i> and <i>qY9</i> for yield and related traits mapped in our study on 5 chromosomes where QTLs for different traits co-localized. The present study demonstrated that <i>O. rufipogon</i> introgressions and QTLs as potential sources associated with positive transgressive variation for enhancement of yield traits.</p>

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Wild species Oryza rufipogon as a potential donor for development of interspecific backcross population in the background of Samba Mahsuri (BPT5204) and detection of yield enhancing QTLs

  • 期刊论文

  • 发表日期：

    2025年11月10日

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  • M. Sridhar,
  • C. Gireesh,
  • Divya Balakrishnan,
  • M. Sujatha,
  • M. S. Anantha,
  • C. H. Raveendra,
  • P. Senguttuvel,
  • D. Srinivasa Chary,
  • V. P. Bhadana,
  • R. M. Sundaram

摘要

The present rice cultivars have reached a yield plateau primarily due to the restricted use of germplasm within Oryza sativa cultivars as parental lines in breeding programmes. Wild ancestors and related species comprise a vital source of genetic material to break the yield barrier. The present investigation was carried out for identification and introgression of yield enhancing QTLs by developing 190 BC2F2 backcross population derived from a cross between Samba Mahsuri*2/O. rufipogon (IRGC106106). Polymorphic SSR markers spanning the genome were used to genotype the mapping population to construct a linkage map of 1893 cM (Kosambi). The CIM identified a total of 47 significant QTLs at P < 0.001 (LOD > 2.50) for ten yield traits distributed across 11 chromosomes. Among these, O. rufipogon alleles contributed a beneficial effect on 22 QTLs. The cumulative phenotypic variance explained by donor-derived QTLs for target traits was ranging from 2.12 to 32.65% with a single major QTL qPL9.1 with 29.14% PVE. Yield increase in introgression lines over Samba Mahsuri (BPT5204) was attributed by beneficial allele from O. rufipogon for the traits such as productive tillers, panicle length, test weight and grain yield per plant. Three major QTLs with more than 10% phenotypic variance observed. Significant genomic region qPL9.1 was detected from O. rufipogon associated with panicle length, with 29.14% of observed phenotypic variance. qDF1.1 and qPH2.1 are the other major QTLs from recurrent parent Samba Mahsuri with 29.33% and 29.20% phenotypic variance respectively. There were six QTL hotspots viz., qY1a, qY1b, qY2, qY3, qY7 and qY9 for yield and related traits mapped in our study on 5 chromosomes where QTLs for different traits co-localized. The present study demonstrated that O. rufipogon introgressions and QTLs as potential sources associated with positive transgressive variation for enhancement of yield traits.