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<p>The spike length (SL) in wheat (<i>Triticum aestivum</i> L.) is one of the most essential component traits for grain yield. Thus, identifying genomic regions associated with SL could advance molecular breeding for grain yield through dissecting the genetic basis of spike development in wheat. The current investigation developed a linkage map for SL using a set of 92 F<sub>2:16</sub> RILs, which were genotyped using the 35K SNP array. QTL mapping for SL revealed 11 QTLs on chromosomes 1D, 3B, 3D, 4A, 5A, 6B, 6D, and 7B. A stable major QTL, <i>Qsl.nhv-4A.a</i> (LOD: 5.77–7.61; R<sup>2</sup>: 23.20–28.66%) flanked by SNP markers, AX-94411546 and AX-94916062 (4A:715307939–722410554) was detected at 4.18&#xa0;cM interval on 4AS chromosome. The confidence intervals of <i>Qsl.nhv-4A.a</i> revealed six candidate genes with functional domains of known orthologs for SL. Structural and functional annotation further revealed that <i>TraesCS4A02G451300</i>, <i>TraesCS4A02G452000, TraesCS4A02G452300,</i> and <i>TraesCS4A02G453000</i> are the orthologs of <i>Larger Panicle 1 (LP1)</i>, whereas TraesCS4A02G455700 is an ortholog of <i>Aberrant Panicle Organization1 (OsAPO1)</i>. In addition, <i>TraesCS4A02G452700</i> showed close orthology with the <i>Teosinte Branched1</i> (<i>ZmTB1</i>). The expression analysis of candidate SL genes in developing spike tissues suggested their potential role in shaping spike architecture. The gene coexpression analysis further indicated the regulatory and pleiotropic significance of SL candidate genes. Overall, the current investigation has broadened the genetic and molecular understanding of SL, and the identified QTLs and candidate genes could serve as potential targets for improving the grain yield in wheat.</p>

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Quantitative trait loci mapping uncovered the novel and major QTL and candidate genes controlling spike length in bread wheat (Triticum aestivum L.)

  • 期刊论文

  • 发表日期：

    2025年8月14日

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  • Ajay Kumar Chandra,
  • Priyanka Agarwal,
  • Aakriti Verma,
  • Ujjwal Kapoor,
  • Hemant Sharma,
  • Niharika Mallick,
  • Niranjana Murukan,
  • K. Raghunandan,
  • Lekshmy Sathee,
  • Mallana Gowdra Mallikarjuna,
  • Shailendra Kumar Jha,
  • Vinod

摘要

The spike length (SL) in wheat (Triticum aestivum L.) is one of the most essential component traits for grain yield. Thus, identifying genomic regions associated with SL could advance molecular breeding for grain yield through dissecting the genetic basis of spike development in wheat. The current investigation developed a linkage map for SL using a set of 92 F2:16 RILs, which were genotyped using the 35K SNP array. QTL mapping for SL revealed 11 QTLs on chromosomes 1D, 3B, 3D, 4A, 5A, 6B, 6D, and 7B. A stable major QTL, Qsl.nhv-4A.a (LOD: 5.77–7.61; R2: 23.20–28.66%) flanked by SNP markers, AX-94411546 and AX-94916062 (4A:715307939–722410554) was detected at 4.18 cM interval on 4AS chromosome. The confidence intervals of Qsl.nhv-4A.a revealed six candidate genes with functional domains of known orthologs for SL. Structural and functional annotation further revealed that TraesCS4A02G451300, TraesCS4A02G452000, TraesCS4A02G452300, and TraesCS4A02G453000 are the orthologs of Larger Panicle 1 (LP1), whereas TraesCS4A02G455700 is an ortholog of Aberrant Panicle Organization1 (OsAPO1). In addition, TraesCS4A02G452700 showed close orthology with the Teosinte Branched1 (ZmTB1). The expression analysis of candidate SL genes in developing spike tissues suggested their potential role in shaping spike architecture. The gene coexpression analysis further indicated the regulatory and pleiotropic significance of SL candidate genes. Overall, the current investigation has broadened the genetic and molecular understanding of SL, and the identified QTLs and candidate genes could serve as potential targets for improving the grain yield in wheat.