Modified slag amendment enhances growth, antioxidant defence and yield of spinach (SpinaceaOleracea L.) under cadmium stress in vertisol
摘要
A study was performed to examine the effect of slag and modified slag on plant physiological parameters, soil properties and growth under cadmium (Cd) contamination in spinach. Bulk soil sample of Vertisol (Typic Haplustert) were artificially contaminated with cadmium nitrate at 20 mg Cd kg− 1 soil. The soil contaminated with Cd was amended with untreated slag, slag modified with acid (AM slag), slag modified with spent wash (SWM slag), and spent wash (SW) alone. The Selection-1 variety of spinach crop was grown under greenhouse condition for 60 days. Results indicated significant reductions in spinach dry matter yield, leaf area and photosynthetic pigments due to Cd stress, resulting in decrease of 36.2% in leaf dry matter yield, 62.9% in root dry matter yield, and 37.9% in leaf area. Amendments, particularly SWM slag, improved spinach growth, increased leaf and root dry matter yields by 50.8% and 131.4%, respectively, and leaf area by 37.4% compared to unamended Cd-contaminated soil. Chlorophyll a, b, and carotenoids contents significantly improved with SWM slag, showing increases of 32.8%, 69.3%, and 80.5%, respectively. Soil properties also improved with amendments, showing increased dehydrogenase activity, particularly in SWM slag (5.03 µg TPF g− 1 soil h− 1) and reduced bioavailable Cd content by 39.3% due to formation of metal-silicate complexes. Furthermore, superoxide dismutase (SOD) and catalase (CAT) activities were enhanced in SWM slag-amended soils, indicating improved antioxidant defense mechanisms in spinach. The study clearly demonstrates the potential of SWM slag in mitigating Cd toxicity and enhancing spinach growth and soil properties.