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<p>Water-soluble carbohydrates (WSCs) serve as a potential buffer for grain filling in wheat, when current leaf photosynthesis is inhibited by abiotic stress. The potential of genotype to store WSCs for its remobilisation is determined by its stem-specific weight i.e. stem density. To examine the extent to which mobilisation of carbon reserve occurs under multi-environment conditions and the genomic regions associated with stem density in wheat, a field experiment was conducted at Indian Agricultural Research Institute (IARI), New Delhi, India with 220 wheat RILs developed by crossing HD3086 and HI1500 under control, drought, heat and combined stress (heat and drought) conditions. Genotyping of population (21 days seedling) was done with 35&#xa0;K Wheat Breeder Array followed by QTL mapping with inclusive composite interval mapping (ICIM) software. Selection of superior lines were carried out by using combined approach of multi-trait genotype-ideotype distance index (MGIDI), factor analysis and genotype-ideotype distance (FAI-BLUP) and Smith-Hazel index (SH). In our study, total 9 quantitative traits loci (QTLs) were mapped, which were linked to stem density (peduncle, penultimate and lower internode) with LOD score &gt; 3.5, of which 7 were mapped as major QTLs. In-silico gene expression analysis revealed various important genes like sugar transport protein MST4, PPR containing protein, trehalose phosphate synthase, NRT1/PTR FAMILY 8.3-like etc., which are probably involved in maintaining stem density in wheat. Moreover, four lines (HDHI-12, HDHI-87, HDHI-142 and HDHI-194) were identified as superior lines, which can be used as potential donors to elite wheat cultivars. Our study shed light on genetic basis of regulation of stem density in wheat, which accelerates the possible marker-assisted and genomic selection for higher stem density and stem reserve mobilisation.</p>

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Mapping of the QTLs governing stem-specific weight for stem reserve mobilisation in wheat (Triticum aestivum L.) under combined heat and drought stress

  • 期刊论文

  • 发表日期：

    2025年2月5日

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  • Sukumar Taria,
  • Ajay Arora,
  • Hari Krishna,
  • Karthik Kumar Manjunath,
  • Sudhir Kumar,
  • Biswabiplab Singh,
  • Shashi Meena,
  • Animireddy China Malakondaiah,
  • S. Kousalya,
  • Jasdeep Chatrath Padaria,
  • Pradeep Kumar Singh,
  • Badre Alam,
  • Sushil Kumar,
  • Ayyanadar Arunachalam

摘要

Water-soluble carbohydrates (WSCs) serve as a potential buffer for grain filling in wheat, when current leaf photosynthesis is inhibited by abiotic stress. The potential of genotype to store WSCs for its remobilisation is determined by its stem-specific weight i.e. stem density. To examine the extent to which mobilisation of carbon reserve occurs under multi-environment conditions and the genomic regions associated with stem density in wheat, a field experiment was conducted at Indian Agricultural Research Institute (IARI), New Delhi, India with 220 wheat RILs developed by crossing HD3086 and HI1500 under control, drought, heat and combined stress (heat and drought) conditions. Genotyping of population (21 days seedling) was done with 35 K Wheat Breeder Array followed by QTL mapping with inclusive composite interval mapping (ICIM) software. Selection of superior lines were carried out by using combined approach of multi-trait genotype-ideotype distance index (MGIDI), factor analysis and genotype-ideotype distance (FAI-BLUP) and Smith-Hazel index (SH). In our study, total 9 quantitative traits loci (QTLs) were mapped, which were linked to stem density (peduncle, penultimate and lower internode) with LOD score > 3.5, of which 7 were mapped as major QTLs. In-silico gene expression analysis revealed various important genes like sugar transport protein MST4, PPR containing protein, trehalose phosphate synthase, NRT1/PTR FAMILY 8.3-like etc., which are probably involved in maintaining stem density in wheat. Moreover, four lines (HDHI-12, HDHI-87, HDHI-142 and HDHI-194) were identified as superior lines, which can be used as potential donors to elite wheat cultivars. Our study shed light on genetic basis of regulation of stem density in wheat, which accelerates the possible marker-assisted and genomic selection for higher stem density and stem reserve mobilisation.