<p>Resin from conifers is a sustainable material of human interest. Strategies to increase resin production, including chemical stimulation, are being implemented. This procedure is used in Brazil, India, and Indonesia, dominating countries in global resin production. China and Mexico are also recognized as crucial resin producers. The prime resin production zone in Mexico is Michoacan state, where nearly 90% of Mexican resin is produced, and the native <i>Pinus devoniana</i> var. <i>cornuta</i> and <i>Pinus pringlei</i> are included in the preferred species for this economic activity. Interestingly, chemical stimulation to improve the resin production of these species has not been applied. Consequently, this research aims to evaluate for the first time using chemical stimulation to increase the resin production from <i>P. pringlei</i> and <i>P. devoniana</i> var. <i>cornuta</i>. The stimulation was promoted by applying aqueous benzoic acid/H₂SO₄ directly on wounds. Analysis of Variance and Multiple Factor Analysis were performed to relate resin yield with chemical stimulation. Climatic factors were also involved in the study, including temperature, radiation, relative humidity, and wind. The influence of chemical stimulation on resin canals and the chemical composition of turpentine was boarded. Results provided exciting conclusions about the relationship between chemical stimulation, environmental impact, and anatomical changes in resin canals on the resination process in pines; in consequence, the chemical stimulation resulted in a potential strategy to increase resin production using <i>P. pringlei</i> and <i>P. devoniana</i> var. <i>cornuta</i> from Mexico.</p>