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<p><i>Pandanus amaryllifolius</i> Roxb. is an ecologically and economically important plant, yet its response to environmental stresses, such as drought, remains poorly understood because of limited genetic and molecular research. This knowledge gap hinders efforts to develop drought-resistant cultivars. To address this, we investigated the transcriptomic response of <i>P. amaryllifolius</i>, to drought stress through differential gene expression analysis. Plants were subjected to drought treatment for 7 and 14&#xa0;days after water withdrawal. De novo transcriptome assembly produced 257,695 transcripts, while differential gene expression analysis identified 4,589 and 9,494 differentially expressed genes (DEGs) at 7 and 14&#xa0;days, respectively, compared to controls. Gene Ontology enrichment analysis revealed DEGs involved in DNA-binding transcription factor activity, transporter activity, and carbohydrate metabolism. Cellular changes involving the cell wall and plasma membrane were also detected. KEGG pathway analysis highlighted phenylpropanoid biosynthesis, starch and sucrose metabolism, flavonoid biosynthesis, plant hormone signal transduction, and the MAPK signaling pathway. Validation of the 17 selected transcripts showed a strong positive correlation (<i>p</i> &lt; 0.05) between RNA sequencing and NanoString nCounter results. This study provides the first comprehensive transcriptomic profile of drought-stressed <i>P. amaryllifolius</i>, uncovering critical molecular pathways involved in drought adaptation and offering valuable genetic insights for future breeding programs.</p>

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Pandanus amaryllifolius transcriptome under drought stress reveals differential expression profile of genes related to plant hormone signal transduction and MAPK signaling pathways

  • 期刊论文

  • 发表日期：

    2025年3月15日

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  • Sidek Norhafizah,
  • Boon Chin Tan,
  • Taheri Sima,
  • Mebus Katharina,
  • Chee How Teo

摘要

Pandanus amaryllifolius Roxb. is an ecologically and economically important plant, yet its response to environmental stresses, such as drought, remains poorly understood because of limited genetic and molecular research. This knowledge gap hinders efforts to develop drought-resistant cultivars. To address this, we investigated the transcriptomic response of P. amaryllifolius, to drought stress through differential gene expression analysis. Plants were subjected to drought treatment for 7 and 14 days after water withdrawal. De novo transcriptome assembly produced 257,695 transcripts, while differential gene expression analysis identified 4,589 and 9,494 differentially expressed genes (DEGs) at 7 and 14 days, respectively, compared to controls. Gene Ontology enrichment analysis revealed DEGs involved in DNA-binding transcription factor activity, transporter activity, and carbohydrate metabolism. Cellular changes involving the cell wall and plasma membrane were also detected. KEGG pathway analysis highlighted phenylpropanoid biosynthesis, starch and sucrose metabolism, flavonoid biosynthesis, plant hormone signal transduction, and the MAPK signaling pathway. Validation of the 17 selected transcripts showed a strong positive correlation (p < 0.05) between RNA sequencing and NanoString nCounter results. This study provides the first comprehensive transcriptomic profile of drought-stressed P. amaryllifolius, uncovering critical molecular pathways involved in drought adaptation and offering valuable genetic insights for future breeding programs.