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<p><i>FKF1</i> (<i>Flavin binding, kelch repeat, F-box 1</i>) was reported functions in circadian clock and flowering, but the function of <i>BjuFKF1</i> has not been characterized. To identify its functions, <i>BjuFKF1</i> was ectopic overexpressed in <i>Arabidopsis</i>. At last, three independent <i>BjuFKF1</i> overexpression (OE) lines were obtained, which displayed early flowering and more branches. Further study found that exogenous GA up-regulated <i>FKF1</i> mRNA expression at seedling, reproductive stages and under both long day and blue light. qRT-PCR assays indicated that all GA related genes were up-regulated in <i>BjuFKF1</i> OE line, exogenous GA reduced most GA related genes under short day but up-regulated under medium day. When under different lights, only GA catabolism and GA response genes were up-regulated. Exogenous GA increased most GA related genes under blue light and green light, but reduced under red light. All the results indicated that <i>BjuFKF1</i> involves in GA signal pathway, functions in flowering time and branches regulation.</p>

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Ectopic overexpression of a plant-specific LOV blue light receptor gene from B. juncea in A. thaliana promotes flowering and branching

  • 期刊论文

  • 发表日期：

    2025年3月17日

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  • Xin Dai,
  • Yawen Zhou,
  • Wenbo Li,
  • Yuanqing lu,
  • Liang Zhao,
  • Zijing Xu,
  • Daqing Tian,
  • Luyun Luo,
  • Xinrui Cao,
  • Jing Zeng,
  • Yanling Mo

摘要

FKF1 (Flavin binding, kelch repeat, F-box 1) was reported functions in circadian clock and flowering, but the function of BjuFKF1 has not been characterized. To identify its functions, BjuFKF1 was ectopic overexpressed in Arabidopsis. At last, three independent BjuFKF1 overexpression (OE) lines were obtained, which displayed early flowering and more branches. Further study found that exogenous GA up-regulated FKF1 mRNA expression at seedling, reproductive stages and under both long day and blue light. qRT-PCR assays indicated that all GA related genes were up-regulated in BjuFKF1 OE line, exogenous GA reduced most GA related genes under short day but up-regulated under medium day. When under different lights, only GA catabolism and GA response genes were up-regulated. Exogenous GA increased most GA related genes under blue light and green light, but reduced under red light. All the results indicated that BjuFKF1 involves in GA signal pathway, functions in flowering time and branches regulation.