Purpose <p>This study aims to evaluate the knowledge and first-aid practices of Greek school nurses regarding dental trauma.</p> Methods <p>A cross-sectional study, utilizing a researcher-developed questionnaire based on the relevant literature, was conducted. Questions about demographic characteristics, work experience, and knowledge of first aid for dental injuries were included<i>.</i> Three dental trauma case scenarios of anterior teeth, including crown fracture, lateral luxation, and avulsion were included. The results were statistically analyzed using SPSS at <i>p</i> &lt; 0.05%.</p> Results <p>A total of 136 school nurses from various regions of Greece responded. The majority of participants (77.2%) had worked as school nurses for fewer than 5&#xa0;years, and fewer than 35% of the respondents received any training related to dental trauma. When confronted with a crown fracture, most indicated that the tooth was permanent, and 60.3% would place the tooth fragment in liquid for preservation. In the case of lateral luxation, the most common response was to contact the child’s parents. For avulsion, half of the respondents were unaware of the importance of timing; the majority would choose an appropriate transfer medium and refer the patient to a dentist.</p> Conclusions <p>Greek school nurses have limited capability to manage dental trauma cases, and their current level of knowledge is insufficient. Current management of dental trauma in schools often deviates from recommended guidelines.</p>