Seed germination response of the invasive Haloxylon persicum in Tunisia

Biological invasion represents a major worldwide threat to native biodiversity and environmental stability. Haloxylon persicum was introduced to Tunisia (North Africa) with Saharan bioclimate in 1969 to fix sandy dunes. Since then, it has gained significant interest for its potential to colonize, proliferate, and become naturalized in Tunisia. Hence, understanding the seed germination response of H. persicum to abiotic conditions, including temperature, water stress, and salt stress, is crucial for predicting its future spread and adopting effective control strategies. Our work investigated the germination behavior of this invasive plant species by incubation at temperatures from 10.0°C to 35.0°C and at various osmotic potentials (−2.00, −1.60, −1.00, −0.50, and 0.00 MPa) of polyethylene glycol-6000 (PEG₆₀₀₀, indicating water stress) and sodium chloride (NaCl, indicating salt stress) solutions. Results showed remarkable correlations among the seed functional traits of H. persicum, indicating adaptive responses to local environmental constraints. The maximum germination rate was recorded at 25.0°C with a rate of 0.39/d. Using the thermal time model, the base temperature was recorded at 8.4°C, the optimal temperature was 25.5°C, and the ceiling temperature was found at 58.3°C. Besides, based on the hydrotime model, the base water potential showed lower values of −7.74 and −10.90 MPa at the optimal temperatures of 25.0°C and 30.0°C, respectively. Also, the species was found to have excellent tolerance to drought (water stress) compared to salt stress, which has implications for its potential growth into new habitats under climate change. Combining ecological and physiological approaches, this work elucidates the invasive potential of H. persicum and contributes to the protection of species distribution in Tunisian ecosystems.