Let \(h: E(G) \rightarrow [0,1]\) be an edge indicator function of a graph G. For \(k \in \mathbb {N}\) , a fractional k-factor in G is a spanning subgraph induced by \(F_{h}=\{e \in E(G): h(e)>0\}\) if \(\sum _{e \in E_{G}(v)}h(e)=k\) for every vertex \(v \in V(G)\) , where \(E_{G}(v)\) denotes the set of edges incident with v in G. We say G has a fractional k-factor if such an edge indicator function h exists. A graph G is a fractional (k, l)-critical graph if, after deleting any l vertices from G, the resulting subgraph still admits a fractional k-factor. It is interesting to determine whether a graph is fractional (k, l)-critical. In this paper, we establish some sufficient conditions involving the size and the spectral radius to guarantee that a graph G with minimum degree \(\delta (G)\) is fractional (k, l)-critical, which generalize the results on fractional k-factors due to Li et al. (Linear Algebra Appl 715:32–45, 2025).