Scholarlink

<p>Ultra-sensitive, versatile, and contamination-resistant nucleic acid detection remains a major challenge in molecular diagnostics, particularly for applications requiring simultaneous pathogen screening and precise genotyping. Herein, we report a programmable, label-free isothermal sensing strategy termed probe-initiated amplification and transcription for high-sensitivity sensing platform (PATSP), which decouples target recognition from signal generation <i>via</i> a universal signal-transduction architecture. In this system, target-specific hybridization events are converted into a universal intermediate trigger that activates a fixed amplification-transcription cascade, generating malachite green (MG) RNA aptamers as the fluorescent output. By integrating loop-mediated isothermal amplification (LAMP) with <i>in situ</i> transcription in a single closed-tube format, PATSP achieves high sensitivity while effectively suppressing aerosol contamination and false-positive signals. Notably, only minimal probe sequence replacement is required for new targets, enabling one-tube multiplex screening using a single primer pair without primer cross-reactivity. Under optimized conditions, PATSP enables attomolar-level detection of multiple human papillomavirus (HPV) genotypes (11, 16, 18, and 52) and human immunodeficiency virus (HIV) over a wide dynamic range. Clinical validation using cervical secretion samples showed 100% concordance with quantitative polymerase chain reaction (qPCR) for both HPV screening and genotyping. Collectively, PATSP provides a robust and broadly applicable platform for nucleic acid diagnostics and point-of-care testing.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Ultra-sensitive One-step Platform for Logic Screening and Detecting of HPV Genotypes via a Programmable Amplification-transcription Cascade

  • 期刊论文

  • 发表日期：

    2026年3月17日

查看全文
  • Zilin Tian,
  • Lijuan Qi,
  • Lingxuan Wang,
  • Tianshi Yang,
  • Yan Du

摘要

Ultra-sensitive, versatile, and contamination-resistant nucleic acid detection remains a major challenge in molecular diagnostics, particularly for applications requiring simultaneous pathogen screening and precise genotyping. Herein, we report a programmable, label-free isothermal sensing strategy termed probe-initiated amplification and transcription for high-sensitivity sensing platform (PATSP), which decouples target recognition from signal generation via a universal signal-transduction architecture. In this system, target-specific hybridization events are converted into a universal intermediate trigger that activates a fixed amplification-transcription cascade, generating malachite green (MG) RNA aptamers as the fluorescent output. By integrating loop-mediated isothermal amplification (LAMP) with in situ transcription in a single closed-tube format, PATSP achieves high sensitivity while effectively suppressing aerosol contamination and false-positive signals. Notably, only minimal probe sequence replacement is required for new targets, enabling one-tube multiplex screening using a single primer pair without primer cross-reactivity. Under optimized conditions, PATSP enables attomolar-level detection of multiple human papillomavirus (HPV) genotypes (11, 16, 18, and 52) and human immunodeficiency virus (HIV) over a wide dynamic range. Clinical validation using cervical secretion samples showed 100% concordance with quantitative polymerase chain reaction (qPCR) for both HPV screening and genotyping. Collectively, PATSP provides a robust and broadly applicable platform for nucleic acid diagnostics and point-of-care testing.