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<p><sup>19</sup>F NMR chemical shift-switching probes are highly effective tools for investigating complex biological processes, primarily due to their capacity to produce distinct, crosstalk-free signals from multiple biomarkers. Capitalizing on this capability, we have developed two sets of <sup>19</sup>F NMR probes specifically designed for identifying senescence-associated <i>β</i>-galactosidase (<i>β</i>-gal) and monoamine oxidase A (MAO-A). The probes <b>G-3F</b> and <b>G-F</b> were engineered for <i>β</i>-gal detection, while <b>M-3F, M-2F</b>, and <b>M-F</b> were tailored for MAO-A detection. Among them, probes <b>G-F</b> and <b>M-F</b> demonstrated superior chemical shift changes relative to their series counterparts: <b>G-F</b>’s <sup>19</sup>F signal shifted from <i>δ</i> −77.8 to <i>δ</i> −81.8 after reaction with <i>β</i>-gal, while <b>M-F</b>’s signal shifted from <i>δ</i> −115.7 to <i>δ</i> −109.2 upon encountering MAO-A. Crucially, the resulting signals are well-separated, which facilitates simultaneous detection without mutual interference, as successfully verified in senescent cell models.</p>

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19F NMR Chemical Shift-switching Probes Enabling Simultaneous Detection of Senescence-associated β-Galactosidase and Monoamine Oxidase A

  • 期刊论文

  • 发表日期：

    2026年1月5日

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  • Ruimin Xia,
  • Yu Zeng,
  • Yumeng Lu,
  • Jingyu Wang,
  • Ling Li,
  • Xiangjie Luo,
  • Yong Qian

摘要

19F NMR chemical shift-switching probes are highly effective tools for investigating complex biological processes, primarily due to their capacity to produce distinct, crosstalk-free signals from multiple biomarkers. Capitalizing on this capability, we have developed two sets of 19F NMR probes specifically designed for identifying senescence-associated β-galactosidase (β-gal) and monoamine oxidase A (MAO-A). The probes G-3F and G-F were engineered for β-gal detection, while M-3F, M-2F, and M-F were tailored for MAO-A detection. Among them, probes G-F and M-F demonstrated superior chemical shift changes relative to their series counterparts: G-F’s 19F signal shifted from δ −77.8 to δ −81.8 after reaction with β-gal, while M-F’s signal shifted from δ −115.7 to δ −109.2 upon encountering MAO-A. Crucially, the resulting signals are well-separated, which facilitates simultaneous detection without mutual interference, as successfully verified in senescent cell models.