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<p>In this study, Pepsin@AuNPs (Pep@AuNPs) and Trypsin@AuNPs (Try@AuNPs) were synthesized by a microfluidic droplet system using Pepsin and Trypsin as protection reagents and NaOH as reducing reagents. Compared to the synthesis method in a flask, the AuNPs synthesized by the microfluidic droplet system demonstrated uniform nucleation, superior ultraviolet absorption performance, high stability and short preparation cycles (15 min). The detection range of Cu(II) by Pep@AuNPs was 1.0–100.0 µmol/L and the detection limit was 0.3 µmol/L. The detection range of <i>L</i>-Cysteine by Try@AuNPs was 0.3–250.0 mmol/L and the detection limit was 0.1 mmol/L. This universal method provides an effective strategy for the detection of bioactive molecules, such as metal ions and amino acids by AuNPs with protein as a protective agent.</p>

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Protein@AuNPs Synthesized by Microfluidic Droplet System and Application in Cu(II) and L-Cysteine Sensing

  • 期刊论文

  • 发表日期：

    2025年1月15日

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  • Xiaotong Zhu,
  • Xinyu Li,
  • Li Qi,
  • Rongyue Zhang,
  • Nan Li,
  • Xiaonan He,
  • Juan Qiao

摘要

In this study, Pepsin@AuNPs (Pep@AuNPs) and Trypsin@AuNPs (Try@AuNPs) were synthesized by a microfluidic droplet system using Pepsin and Trypsin as protection reagents and NaOH as reducing reagents. Compared to the synthesis method in a flask, the AuNPs synthesized by the microfluidic droplet system demonstrated uniform nucleation, superior ultraviolet absorption performance, high stability and short preparation cycles (15 min). The detection range of Cu(II) by Pep@AuNPs was 1.0–100.0 µmol/L and the detection limit was 0.3 µmol/L. The detection range of L-Cysteine by Try@AuNPs was 0.3–250.0 mmol/L and the detection limit was 0.1 mmol/L. This universal method provides an effective strategy for the detection of bioactive molecules, such as metal ions and amino acids by AuNPs with protein as a protective agent.