Scholarlink

Background <p>Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most frequent chronic liver disease. The literature strongly links MASLD to increased epicardial fat thickness, left ventricular hypertrophy, and valvular calcifications. The effects of <i>Lactobacillus casei</i> and <i>Chlorella vulgaris</i> microalgae on MASLD prevention are good. NF-kB, Nrf-2, and cardiac tissue structural alterations in C57BL/6 mice produced MASLD were studied.</p> Methods and results <p>Forty adult male C57BL/6 mice were allocated to five groups: control (CTL, standard chow), MASLD (high fat diet for 6 weeks), MASLD + <i>Chlorella vulgaris</i> (150&#xa0;mg/kg), and MASLD + <i>Lactobacillus casei</i> (10<sup>8</sup> CFU/ml). Oral gavage needle-treated mice for 35 days. After blood drawing, dissection, and preparation, heart tissue stereology was assessed. Real-time RT PCR was used to analyze <i>NF-kB</i> and <i>Nrf-2</i> gene expression, and calorimetric methods were used to measure SOD, GPX, catalase, TAC, MDA, and LDH enzyme activity. <i>NF-kB</i> gene expression, ALT, LDH, AST enzyme activity, MDA concentration, left ventricle volume, cardiomyocyte volume, connective tissue volume, and capillary length increased significantly (<i>p</i> &lt; 0.05). The treated groups showed significant decreases in <i>NF-kB</i> gene expression, ALT, LDH, AST, enzymes, MDA concentration, left ventricle volume, cardiomyocyte volume, connective tissue volume, capillary length, <i>Nrf-2</i> gene expression, and SOD, GPX, and TAC enzyme activity (<i>p</i> &lt; 0.05).</p> Conclusion <p>The present study showed that MASLD causes structural changes in the heart tissue to some extent. Also, administration of <i>Chlorella vulgaris</i> and <i>Lactobacillus casei</i> can reduce complications caused by MASLD.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Protective effects of Lactobacillus casei and Chlorella vulgaris on cardiac gene expression (NF-κB, Nrf-2) and structural alterations in a C57BL/6 mouse model of MASLD

  • 期刊论文

  • 发表日期：

    2025年11月5日

查看全文
  • Melika Roueintan,
  • Farhad Koohpeyma,
  • Arzhang Naseri,
  • Ali Mahdavi,
  • Haniyeh Keyghobadi,
  • Amir Reza Karamibonari,
  • Sanaz Dastghaib

摘要

Background

Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most frequent chronic liver disease. The literature strongly links MASLD to increased epicardial fat thickness, left ventricular hypertrophy, and valvular calcifications. The effects of Lactobacillus casei and Chlorella vulgaris microalgae on MASLD prevention are good. NF-kB, Nrf-2, and cardiac tissue structural alterations in C57BL/6 mice produced MASLD were studied.

Methods and results

Forty adult male C57BL/6 mice were allocated to five groups: control (CTL, standard chow), MASLD (high fat diet for 6 weeks), MASLD + Chlorella vulgaris (150 mg/kg), and MASLD + Lactobacillus casei (108 CFU/ml). Oral gavage needle-treated mice for 35 days. After blood drawing, dissection, and preparation, heart tissue stereology was assessed. Real-time RT PCR was used to analyze NF-kB and Nrf-2 gene expression, and calorimetric methods were used to measure SOD, GPX, catalase, TAC, MDA, and LDH enzyme activity. NF-kB gene expression, ALT, LDH, AST enzyme activity, MDA concentration, left ventricle volume, cardiomyocyte volume, connective tissue volume, and capillary length increased significantly (p < 0.05). The treated groups showed significant decreases in NF-kB gene expression, ALT, LDH, AST, enzymes, MDA concentration, left ventricle volume, cardiomyocyte volume, connective tissue volume, capillary length, Nrf-2 gene expression, and SOD, GPX, and TAC enzyme activity (p < 0.05).

Conclusion

The present study showed that MASLD causes structural changes in the heart tissue to some extent. Also, administration of Chlorella vulgaris and Lactobacillus casei can reduce complications caused by MASLD.