Background <p>Cleaning activities are critical in pharmaceutical manufacturing to prevent cross-contamination of Active Pharmaceutical Ingredients (APIs). Traditionally, cleaning validation protocols have focused on production lines. However, there is a growing trend toward extending these protocols to Quality Control (QC) laboratories, encompassing both glassware and stainless-steel equipment.</p> Objectives <p>This paper presents a systematic approach for developing cleaning validation protocols specifically designed for QC laboratory equipment, aimed at improving cleaning effectiveness and ensuring regulatory compliance.</p> Methods <p>The proposed methodology includes: (i) identifying the worst-case API; (ii) performing recovery studies to optimize sampling methods and solvent selection; and (iii) employing statistical tools such as descriptive analysis and hypothesis testing to refine the protocol in line with current industry standards.</p> Results <p>A case study involving Oxcarbazepine demonstrates the application of the proposed protocol, evaluating surface contamination across various QC instruments and assessing detergent residues to validate cleaning effectiveness.</p> Conclusion <p>The proposed strategy provides a structured, statistically grounded framework for developing cleaning validation protocols in QC laboratories, promoting effective contamination control and adherence to regulatory standards.</p> Graphical abstract <p></p>