GC–MS Characterization of Organ Specific Phytochemical Diversity in Gardenia gummifera L.f
摘要
Gardenia gummifera L.f. is a medicinally important plant species traditionally employed in Ayurveda, Siddha, and Unani systems. Despite its ethnomedicinal relevance, systematic phytochemical investigations across different plant parts remain limited. The present study aimed to assess the phytochemical diversity of ethanolic extracts from G. gummifera leaf, flower, fruit, and bark using Gas Chromatography–Mass Spectrometry (GC–MS). GC–MS profiling revealed tissue-specific chemical signatures: leaf yielded 14 compounds dominated by methyl abietate (52.39%) and γ-sitosterol (13.20%), flower contained eight compounds including benzoic acid (12.25%) and azulene (5.46%), fruit exhibited 24 compounds enriched in fatty acids such as n-hexadecanoic acid (23.75%) and octadecanoic acid (10.73%), while bark extracts revealed 13 compounds dominated by germanicol (26.21%) and γ-sitosterol (16.11%). Principal Component Analysis (PCA) explained 83.4% of variance, clearly separating plant parts according to dominant metabolite classes, triterpenoids in bark, diterpenoids in leaf, fatty acids in fruit, and aromatics in flower. To the best of our knowledge, this is the first comparative GC–MS study integrating PCA to profile four distinct parts of G. gummifera. These findings provide a biochemical foundation for future phytopharmaceutical and nutraceutical applications.