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<p>The increasing environmental contamination from crude oil spills necessitates the development of effective bioremediation strategies. Crude-oil-degrading bacteria from contaminated soils have shown promise in mitigating these environmental hazards. While various bacterial strains have been studied for their crude oil degradation abilities, detailed characterization and genomic insights into specific isolates are limited, particularly concerning their metabolic versatility and antimicrobial resistance. This study focused on the whole-genome sequence analysis of a bacterial isolate known for its ability to produce biosurfactants and its capability to degrade and tolerate high concentrations of crude oil, as previously examined. Whole-genome analysis of KUD2 was carried out to determine their identity, metabolic capabilities, and resistance mechanisms. The isolate KUD2 was identified as <i>Pseudomonas aeruginosa</i> through 16S rDNA sequencing. It demonstrated significant hydrocarbon degradation capabilities. Whole-genome analysis revealed KUD2’s possession of a diverse set of enzymes for adaptation to crude oil environments, including those involved in glycine betaine and choline metabolism, stress response, quorum sensing, biofilm formation, and hydrocarbon degradation. KUD2 also harbors antimicrobial resistance (AMR) genes such as KatG, APH(3′)-II/APH(3′)-XV, and OXA-50, along with efflux pumps like AcrAB–TolC and MexAB–OprM that contribute to toxin expulsion and enhanced hydrocarbon degradation. <i>P. aeruginosa</i> KUD2 exhibits remarkable metabolic versatility and resilience in crude-oil-contaminated environments, making it a valuable candidate for bioremediation applications. However, the presence of AMR genes highlights the need for continuous monitoring of resistance traits to ensure effective and safe bioremediation practices.</p>

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Whole-Genome Sequencing and Characterization of Crude-Oil-Degrading Pseudomonas aeruginosa KUD2 Isolated from Oil-Contaminated Soil: Insights into Hydrocarbon Degradation and Antimicrobial Resistance Genes

  • 期刊论文

  • 发表日期：

    2025年9月19日

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  • A. T. Ajao,
  • O. K. Jimoh-Hamza

摘要

The increasing environmental contamination from crude oil spills necessitates the development of effective bioremediation strategies. Crude-oil-degrading bacteria from contaminated soils have shown promise in mitigating these environmental hazards. While various bacterial strains have been studied for their crude oil degradation abilities, detailed characterization and genomic insights into specific isolates are limited, particularly concerning their metabolic versatility and antimicrobial resistance. This study focused on the whole-genome sequence analysis of a bacterial isolate known for its ability to produce biosurfactants and its capability to degrade and tolerate high concentrations of crude oil, as previously examined. Whole-genome analysis of KUD2 was carried out to determine their identity, metabolic capabilities, and resistance mechanisms. The isolate KUD2 was identified as Pseudomonas aeruginosa through 16S rDNA sequencing. It demonstrated significant hydrocarbon degradation capabilities. Whole-genome analysis revealed KUD2’s possession of a diverse set of enzymes for adaptation to crude oil environments, including those involved in glycine betaine and choline metabolism, stress response, quorum sensing, biofilm formation, and hydrocarbon degradation. KUD2 also harbors antimicrobial resistance (AMR) genes such as KatG, APH(3′)-II/APH(3′)-XV, and OXA-50, along with efflux pumps like AcrAB–TolC and MexAB–OprM that contribute to toxin expulsion and enhanced hydrocarbon degradation. P. aeruginosa KUD2 exhibits remarkable metabolic versatility and resilience in crude-oil-contaminated environments, making it a valuable candidate for bioremediation applications. However, the presence of AMR genes highlights the need for continuous monitoring of resistance traits to ensure effective and safe bioremediation practices.