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<p><i>Piper sarmentosum</i> Roxb., a medicinally valuable plant from the South Andaman Islands, is rich in myristicin, a bioactive compound with anticancer potential, making its efficient propagation essential. However, phenolic oxidation remains a major limitation in in vitro culture, leading to tissue browning that inhibits regeneration and often results in explant necrosis. This study demonstrates, for the first time, that internodal explants are superior for micropropagation as they enable simultaneous shoot and root regeneration within a cytokinin-containing medium, eliminating the need for a separate rooting phase. Culturing internodal explants on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BA), kinetin (KIN), or 2-isopentenyl adenine (2iP), at concentrations ranging from 2 to 20&#xa0;µM, revealed that BA at 8&#xa0;µM yielded the highest shoot (3.80 ± 0.11) and root (8.20 ± 0.12) regeneration, but oxidative browning restricted further growth. Histological and stereomicroscopic analyses confirmed phenolic deposition, reinforcing the role of oxidative stress in limiting organogenesis. To counteract this, ascorbic acid (1–12&#xa0;µM) was incorporated into the optimized BA medium, effectively mitigating browning by scavenging reactive oxygen species (ROS) and preventing phenolic oxidation. Additionally, ascorbic acid enhanced cytokinin-mediated cell division and differentiation, resulting in a threefold increase in shoot formation (9.20 ± 0.15) and a fivefold enhancement in root development (42.40 ± 0.15). The substantial increase in root formation provided better anchorage and nutrient absorption, leading to a 100% survival rate, whereas BA alone, which resulted in fewer roots, showed only 76% survival.</p>

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Prevention of Browning and Enhanced In Vitro Organogenesis Mediated by Ascorbic Acid in Piper sarmentosum Roxb.

  • 期刊论文

  • 发表日期：

    2025年8月12日

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  • S. Stephin,
  • A. Gangaprasad,
  • Sam P. Mathew

摘要

Piper sarmentosum Roxb., a medicinally valuable plant from the South Andaman Islands, is rich in myristicin, a bioactive compound with anticancer potential, making its efficient propagation essential. However, phenolic oxidation remains a major limitation in in vitro culture, leading to tissue browning that inhibits regeneration and often results in explant necrosis. This study demonstrates, for the first time, that internodal explants are superior for micropropagation as they enable simultaneous shoot and root regeneration within a cytokinin-containing medium, eliminating the need for a separate rooting phase. Culturing internodal explants on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BA), kinetin (KIN), or 2-isopentenyl adenine (2iP), at concentrations ranging from 2 to 20 µM, revealed that BA at 8 µM yielded the highest shoot (3.80 ± 0.11) and root (8.20 ± 0.12) regeneration, but oxidative browning restricted further growth. Histological and stereomicroscopic analyses confirmed phenolic deposition, reinforcing the role of oxidative stress in limiting organogenesis. To counteract this, ascorbic acid (1–12 µM) was incorporated into the optimized BA medium, effectively mitigating browning by scavenging reactive oxygen species (ROS) and preventing phenolic oxidation. Additionally, ascorbic acid enhanced cytokinin-mediated cell division and differentiation, resulting in a threefold increase in shoot formation (9.20 ± 0.15) and a fivefold enhancement in root development (42.40 ± 0.15). The substantial increase in root formation provided better anchorage and nutrient absorption, leading to a 100% survival rate, whereas BA alone, which resulted in fewer roots, showed only 76% survival.