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<p>Recent reports on mucormycosis and other mycoses in post-COVID cases highlight the search for new antifungal drug leads due to limitations of existing marketed antifungals. Recently microbial endophytes have emerged as promising substitute for plant secondary metabolites with potent antifungal properties. Bioprospecting of endophytes will also help to conserve the biodiversity of plants. So the aim of study was to explore them for antifungal activity as well as to determine their safety in humans. Twenty-nine fungal and fifteen bacterial endophytes were isolated from <i>Aloe vera</i> leaves by optimizing suitable surface sterilization protocol. Screening of antifungal activity was carried out by cross streak and agar overlay methods. Secondary antifungal activity was carried out by agar well diffusion. Out of all, cell-free supernatant (CFS) of Alo NA L 4 exhibited significant antifungal activity against <i>Candida albicans</i> (MCC 1154) and <i>Aspergillus species (</i>MCC 1074). The characterization of antifungal was done by FTIR and GC–MS which led to identification of 1, 2 dichloro-1-ethoxy ethane, S methyl methane thiosulfonate, disulfide dimethyl. Structural changes in <i>Aspergillus cells</i> were studied by SEM which has shown intense morphological deformities in both hyphae and spores. The endophyte was identified as <i>Bacillus velezensis</i> (Accession number MW356850). The effect of antifungal on viability of MCF-10A was determined by MTT assay, which revealed no cytotoxic effect against normal cells. Thus <i>B. velezensis</i> Alo NA L 4 has the potential to be used as promising antifungal lead source for drug discovery.</p>

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Antifungal Potential and Cytotoxicity Studies on Secondary Metabolites of Bacterial Endophytes Isolated From Aloe Vera

  • 期刊论文

  • 发表日期：

    2025年2月6日

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  • Seemadevi Suresh Kadam,
  • Shivangi Shivraj Kanase

摘要

Recent reports on mucormycosis and other mycoses in post-COVID cases highlight the search for new antifungal drug leads due to limitations of existing marketed antifungals. Recently microbial endophytes have emerged as promising substitute for plant secondary metabolites with potent antifungal properties. Bioprospecting of endophytes will also help to conserve the biodiversity of plants. So the aim of study was to explore them for antifungal activity as well as to determine their safety in humans. Twenty-nine fungal and fifteen bacterial endophytes were isolated from Aloe vera leaves by optimizing suitable surface sterilization protocol. Screening of antifungal activity was carried out by cross streak and agar overlay methods. Secondary antifungal activity was carried out by agar well diffusion. Out of all, cell-free supernatant (CFS) of Alo NA L 4 exhibited significant antifungal activity against Candida albicans (MCC 1154) and Aspergillus species (MCC 1074). The characterization of antifungal was done by FTIR and GC–MS which led to identification of 1, 2 dichloro-1-ethoxy ethane, S methyl methane thiosulfonate, disulfide dimethyl. Structural changes in Aspergillus cells were studied by SEM which has shown intense morphological deformities in both hyphae and spores. The endophyte was identified as Bacillus velezensis (Accession number MW356850). The effect of antifungal on viability of MCF-10A was determined by MTT assay, which revealed no cytotoxic effect against normal cells. Thus B. velezensis Alo NA L 4 has the potential to be used as promising antifungal lead source for drug discovery.