Qualitative Analysis of Few Bioactive Compounds from Ardisia solanacea Roxb., Odisha, India

To maintain the novelty of plant bioactive compounds within several formulations and to delimit usage of adulterants, suitable cultivable alternate substitutes for the same fessing identical medicinal attributes is being searched vigorously. Since enormous scientific validation is available regarding active principle embelin presence in plants under Myrsinaceae family; other Non-RET (rare, endangered and threatened) species viz. Ardisia solanacea Roxb. is aspired for isolation of embelin and allied associates like Quercetin, Epigallocatechin (EGC), Epigallocatechin gallate (EGCG) possibly as a new suitable substitute for Embelia ribes. Benzoquinone, embelin has wide medicament use and hence is excessively harvested from Myrsinaceae plants leading to population decrease and has to be validated from different sources. The present piece of research apportions with quantification of embelin and allied associates from Ardisia solanacea leaf and fruit parts through spectrophotometric and ultra-high performance liquid chromatography (UHPLC) technique, which is the first case report on assessment of different compounds from leaf and fruit parts taking methanol, chloroform, petroleum ether and hexane solvents. The results depicted highest embelin concentration (0.989 ± 0.02%), Quercetin (0.86 ± 0.1%), EGC (0.26 ± 0.01%) and EGCG (0.98 ± 0.01%) in methanol extracted fruit sample, while through UHPLC studies, highest embelin concentration (0.91 ± 0.003%), Quercetin (0.197 ± 0.09%), EGC (0.561 ± 0.004%) and EGCG (0.207 ± 0.08%) were found in chloroform extracted fruit sample. Thin Layer chromatography showed retention factor (Rf) at 0.351 with 97.88% accuracy for embelin. The study depicts suitable and precise method for embelin and allied associates validation from Ardisia solanacea, thereby making it suitable candidate for selection as an alternate against other RET species.