MRSA and VRSA isolated from patients hospitalized in the ICU, NICU and surgical departments of hospitals

Staphylococcus aureus is an important pathogen that causes various infections. In this study, we aimed to survey methicillin-resistant Staphylococcus aureus isolated from patients hospitalized in the ICU, NICU and surgical departments of Tehran University hospitals. A total of 430 clinical samples of the patients were collected and identified as Staphylococcus aureus by phenotype and biotype tests. Out of 430 clinical samples, 117 (27.20%) Staphylococcus aureus were isolated of which 42 (35.89%) were identified as MRSA. The mecA gene was detected in all MRSA. The entire MRSA was 100% sensitive to vancomycin and linezolid. Molecular detection of vanA gene in isolated MRSA showed 2 (4.76%) of them contained vanA gene. The high prevalence of MRSA and its antibiotic resistance pattern to vancomycin can lead to treatment failures, uncontrolled nosocomial infections and mortality. Although the PCR-based detection method is superior to other conventional techniques, a combination of these methods can provide diagnostic accuracy.