<p>Wheat serves as a vital grain, supplying energy, nutrients, and diversity for both humans and animals. However, the adaptability of biotic and abiotic stressors presents a significant threat to wheat production, leading to considerable yield reductions. In light of these challenges, it is crucial to emphasize research that aimed at optimizing fertilizer application to mitigate the risk of lodging stress in various wheat genotypes. This study specifically investigates the traits associated with lodging tolerance in wheat under different nitrogen application rates. It seeks to fill the research gap between the combined effects of fertilizer application and lodging stress on the mechanical and chemical properties of different internodes. For a practical approach, samples of various internodes were collected to measure internode length, diameter, hollowness, and tensile strength, revealing changes in the ratio of lignin monomers-syringyl, guaiacyl, and hydroxyphenyl (S: G:H)-in response to different nitrogen management strategies with the help of FTIR spectroscopy. The results demonstrated that these monomers ratio were main component to enhanced mechanical strength.</p>