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<p>Endophytic bacteria associated with medicinal plants are a crucial part of the plant microbial diversity and they usually colonize host tissues in a non-pathogenic manner. The present study investigates the isolation and screening of endophytic bacteria from the internal foliar tissues (leaves) of <i>Abutilon indicum</i>, <i>Solanum trilobatum</i>, <i>Jatropha gossypifolia</i>, <i>Coccinia grandis</i>, <i>Thespesia populnea</i>, <i>Cucumis maderaspatanus</i>, and <i>Datura metel.</i> The main objective of this study was to isolate and molecularly characterize the bacterial endophytes from the leaves of the selected medicinal plants. A total of 22 bacterial strains were isolated and further subjected to molecular characterization and various biochemical assays. Out of these, 9 representative isolates were chosen for in-depth molecular identification. Identification of bacteria was carried out using 16 S rRNA gene sequencing. Also phylogenetic analysis were performed by Neighbor-Joining method employing MEGA software. Identification of 9 distinct isolates was done including <i>Bacillus albus</i>, <i>Paraclostridium bifermentans</i>, <i>Clostridium bifermentans</i>, <i>Brevundimonas terrae</i>, <i>Bacillus cereus</i>, and <i>Bacillus thuringiensis.</i> The results provide a baseline information about the variety of foliar endophytic bacteria linked to medicinal plants.</p>

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Isolation, Screening and Characterization of Endophytic Bacteria from Medicinal Plants

  • 期刊论文

  • 发表日期：

    2026年5月20日

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  • N. Sowmini,
  • J. Senthil Kumar

摘要

Endophytic bacteria associated with medicinal plants are a crucial part of the plant microbial diversity and they usually colonize host tissues in a non-pathogenic manner. The present study investigates the isolation and screening of endophytic bacteria from the internal foliar tissues (leaves) of Abutilon indicum, Solanum trilobatum, Jatropha gossypifolia, Coccinia grandis, Thespesia populnea, Cucumis maderaspatanus, and Datura metel. The main objective of this study was to isolate and molecularly characterize the bacterial endophytes from the leaves of the selected medicinal plants. A total of 22 bacterial strains were isolated and further subjected to molecular characterization and various biochemical assays. Out of these, 9 representative isolates were chosen for in-depth molecular identification. Identification of bacteria was carried out using 16 S rRNA gene sequencing. Also phylogenetic analysis were performed by Neighbor-Joining method employing MEGA software. Identification of 9 distinct isolates was done including Bacillus albus, Paraclostridium bifermentans, Clostridium bifermentans, Brevundimonas terrae, Bacillus cereus, and Bacillus thuringiensis. The results provide a baseline information about the variety of foliar endophytic bacteria linked to medicinal plants.