Scholarlink

<p>Wheat, an essential crop, experiences detrimental effects of heat stress, causing reduced yield and poor grain quality. The experiment was conducted at Krishi Vigyan Kendra (ICAR-KVK) of Banasthali Vidyapith, Rajasthan, India. Ten wheat genotypes were sown under timely and late- sown conditions to study the effect of terminal heat stress in wheat. This study has designed an <i>In-silico</i> genomic primer for the heat-responsive <i>hsp90</i> gene and used PCR to test it on different wheat genotypes. The primer’s successful detection of genetic variations between these wheat genotypes paved the way to grow a heat-resistant wheat crop. The <i>hsp90</i> gene was analyzed, and a marker on chromosome 6D was developed utilizing the Primer 3 <i>In silico</i> tool. Wheat genotypes HD3090, PBW660, CBW38, DBW359, DBW296, HP1209 and PBW590 showed allelic variations with genotypes HI1544, DBW316, and JKW261 aiding in the development of polymorphic markers in wheat.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Assessment of Allelic Variation in Bread Wheat (Triticum aestivum L.) Genotypes by In silico Designed Genomic Marker Targeting the Heat Shock Protein Gene hsp90

  • 期刊论文

  • 发表日期：

    2026年4月9日

查看全文
  • Sunishtha Mishra,
  • Rashmi Priya,
  • Anukriti Srivastava,
  • Girish Chandra Pandey

摘要

Wheat, an essential crop, experiences detrimental effects of heat stress, causing reduced yield and poor grain quality. The experiment was conducted at Krishi Vigyan Kendra (ICAR-KVK) of Banasthali Vidyapith, Rajasthan, India. Ten wheat genotypes were sown under timely and late- sown conditions to study the effect of terminal heat stress in wheat. This study has designed an In-silico genomic primer for the heat-responsive hsp90 gene and used PCR to test it on different wheat genotypes. The primer’s successful detection of genetic variations between these wheat genotypes paved the way to grow a heat-resistant wheat crop. The hsp90 gene was analyzed, and a marker on chromosome 6D was developed utilizing the Primer 3 In silico tool. Wheat genotypes HD3090, PBW660, CBW38, DBW359, DBW296, HP1209 and PBW590 showed allelic variations with genotypes HI1544, DBW316, and JKW261 aiding in the development of polymorphic markers in wheat.