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<p>The fucoidan-rich extract (FRE) was obtained from the brown macroalga <i>Sargassum tenerrimum</i> following an ethanol-acetone-water extraction method. The <sup>1</sup>H NMR spectrum of FRE indicated the presence of a polysaccharide containing repeating units of fucopyranose. The FTIR spectrum confirmed the presence of O–H and C–H groups of fucose units, C–O of pyranose rings and RO–SO<sub>3</sub>− bond of the sulphate groups in the fucoidan polysaccharide. The antioxidant potential of FRE detected by DPPH radical scavenging assay and NO inhibition assay recorded IC<sub>50</sub> values of 51.07 and 69.68&#xa0;µg/mL respectively. With MTT assay, FRE exhibited a dose-dependent increase in cytotoxic effect on human colorectal adenocarcinoma (Caco-2) cells in vitro, while no cytotoxic effect was noted in murine fibroblast (L929) cells. <i>S. tenerrimum</i> FRE exerts no significant cytotoxicity on normal cells whereas it can effectively reduce the viability of cancerous cells proposing its potential application as a chemotherapeutic agent or as an adjuvant to chemotherapy.</p>

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Fucoidan-rich extract from the edible macroalga Sargassum tenerrimum scavenges reactive oxygen species and suppresses proliferation of human colorectal adenocarcinoma cell lines (Caco-2) in vitro

  • 期刊论文

  • 发表日期：

    2026年4月23日

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  • Aparna Kannan,
  • B. N. Bessy Raj,
  • G. S. Anisha

摘要

The fucoidan-rich extract (FRE) was obtained from the brown macroalga Sargassum tenerrimum following an ethanol-acetone-water extraction method. The 1H NMR spectrum of FRE indicated the presence of a polysaccharide containing repeating units of fucopyranose. The FTIR spectrum confirmed the presence of O–H and C–H groups of fucose units, C–O of pyranose rings and RO–SO3− bond of the sulphate groups in the fucoidan polysaccharide. The antioxidant potential of FRE detected by DPPH radical scavenging assay and NO inhibition assay recorded IC50 values of 51.07 and 69.68 µg/mL respectively. With MTT assay, FRE exhibited a dose-dependent increase in cytotoxic effect on human colorectal adenocarcinoma (Caco-2) cells in vitro, while no cytotoxic effect was noted in murine fibroblast (L929) cells. S. tenerrimum FRE exerts no significant cytotoxicity on normal cells whereas it can effectively reduce the viability of cancerous cells proposing its potential application as a chemotherapeutic agent or as an adjuvant to chemotherapy.