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<p>Evaluation and large-scale production of novel antimicrobials are of immense importance to combat the problems caused by acquired resistance. In the present study, hypersaline bacterium <i>Bacillus</i> strain BGUMS27 isolated from marine salt pan of Goa, India showed production of an antibacterial metabolite (SK1) active against methicillin resistant <i>Staphylococcus aureus</i> (MRSA) and an antifungal metabolite (SK2) active against 7 pathogenic fungi. One-factor-at-a-time (OFAT) and statistical response surface methodology (RSM) approaches were applied to optimize the medium composition which increased the yield of SK 1 by 2-fold and SK 2 by 1.3-fold. Furthermore, SK1 was found to be pressure and thermo stable and retained its activity even after autoclaving; whereas SK2 was found stable over wide range of pH (2 to 12). MALDI-TOF profiles revealed similarity of SK1 to the ‘Macrolactin’ family and SK2 to the ‘Iturin’ family. Hence, this study proved that SK1 and SK2 are exceptionally stable lipopeptides produced by a hypersaline <i>Bacillus</i> sp. which could be potential and effective marine drug.</p>

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Optimisation and Partial Characterization of Anti-microbial Metabolites of a Hypersaline Bacillus sp. Strain BGUMS27 Against Human and Plant Pathogens

  • 期刊论文

  • 发表日期：

    2026年3月30日

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  • Shuvankar Ballav,
  • Manasi Pawaskar,
  • Michelle Fernandes,
  • Savita Kerkar

摘要

Evaluation and large-scale production of novel antimicrobials are of immense importance to combat the problems caused by acquired resistance. In the present study, hypersaline bacterium Bacillus strain BGUMS27 isolated from marine salt pan of Goa, India showed production of an antibacterial metabolite (SK1) active against methicillin resistant Staphylococcus aureus (MRSA) and an antifungal metabolite (SK2) active against 7 pathogenic fungi. One-factor-at-a-time (OFAT) and statistical response surface methodology (RSM) approaches were applied to optimize the medium composition which increased the yield of SK 1 by 2-fold and SK 2 by 1.3-fold. Furthermore, SK1 was found to be pressure and thermo stable and retained its activity even after autoclaving; whereas SK2 was found stable over wide range of pH (2 to 12). MALDI-TOF profiles revealed similarity of SK1 to the ‘Macrolactin’ family and SK2 to the ‘Iturin’ family. Hence, this study proved that SK1 and SK2 are exceptionally stable lipopeptides produced by a hypersaline Bacillus sp. which could be potential and effective marine drug.