Scholarlink

<p>Grape (<i>Vitis vinifera</i>), a species in the <i>Vitaceae</i> family, is a major fruit crop cultivated in diverse climates and used to produce wine, juice, raisins, jams, and fresh grapes. Ongoing climatic changes in recent years have been associated with a shift in the causal agent of grape anthracnose, transitioning from <i>Elsinoe ampelina</i> to different species of <i>Colletotrichum</i> genus. A total of 40 anthracnose infected samples were collected from major grape growing districts of Punjab during 2022–23 with the aim to characterize pathogen associated with grape anthracnose in Punjab, India. Based on various morphological characteristics, the fungus was identified as <i>Colletotrichum</i> spp. For molecular confirmation, the ITS region, actin (<i>ACT</i>) and beta-tubulin2 (<i>TUB2</i>) genes were amplified using primers ITS-6/ITS-4, ACT-512F/ ACT-783R and T1/Bt2b, respectively. PCR amplicons of the three loci (ITS, <i>ACT</i>, and <i>TUB2</i>) were sequenced, and the resulting sequences were submitted to GenBank under the accession numbers PQ821402, PQ826576 and PQ826578 for isolate C 3 and PQ821404, PQ826577 and PQ826579 for isolate C 31, respectively. BLAST results and multigene phylogenetic analyses demonstrated that the isolates obtained in this study are phylogenetically affiliated with <i>Colletotrichum siamense</i>. Consequently, integration of morphological traits, molecular phylogeny, and pathogenicity assays confirmed <i>C. siamense</i> as the causal organism and this also marks the first report of occurrence of <i>C. siamense</i> causing grape anthracnose in Punjab, India.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

First Report of Occurrence of Colletotrichum siamense Causing Grape Anthracnose in Punjab, India

  • 期刊论文

  • 发表日期：

    2026年3月30日

查看全文
  • Harmeen Kaur,
  • Amrinder Kaur,
  • Anita Arora,
  • Harpreet Singh

摘要

Grape (Vitis vinifera), a species in the Vitaceae family, is a major fruit crop cultivated in diverse climates and used to produce wine, juice, raisins, jams, and fresh grapes. Ongoing climatic changes in recent years have been associated with a shift in the causal agent of grape anthracnose, transitioning from Elsinoe ampelina to different species of Colletotrichum genus. A total of 40 anthracnose infected samples were collected from major grape growing districts of Punjab during 2022–23 with the aim to characterize pathogen associated with grape anthracnose in Punjab, India. Based on various morphological characteristics, the fungus was identified as Colletotrichum spp. For molecular confirmation, the ITS region, actin (ACT) and beta-tubulin2 (TUB2) genes were amplified using primers ITS-6/ITS-4, ACT-512F/ ACT-783R and T1/Bt2b, respectively. PCR amplicons of the three loci (ITS, ACT, and TUB2) were sequenced, and the resulting sequences were submitted to GenBank under the accession numbers PQ821402, PQ826576 and PQ826578 for isolate C 3 and PQ821404, PQ826577 and PQ826579 for isolate C 31, respectively. BLAST results and multigene phylogenetic analyses demonstrated that the isolates obtained in this study are phylogenetically affiliated with Colletotrichum siamense. Consequently, integration of morphological traits, molecular phylogeny, and pathogenicity assays confirmed C. siamense as the causal organism and this also marks the first report of occurrence of C. siamense causing grape anthracnose in Punjab, India.