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<p><i>Cleome viscosa</i> L. belonging to family Cleomaceae exhibits extensive phenotypic variability. Plants in the current study were separated into long (LM), intermediate (IM) and short (SM) morphs on the basis of their growth habit and branching pattern. Leaf, root and seed tissues of the three morphs were subjected to preliminary analysis for the presence of various phytochemicals followed by estimating the total phenol and flavonoid concentrations in them using the spectrophotometer. Colorimetric tests for ten bioactives were performed. Of the 10, only 8 (Glycosides, alkaloids, flavonoids, steroids, tannins, phenols, coumarins and terpenoids) were detected. Saponins and anthocyanides were not found across morph, solvent and tissue types. Larger number of bioactives were found in the ethanol extract of LM morph. TPC was recorded highest in seeds (5.47 ± 0.011&#xa0;mg/GAE) and least in roots (1.029 ± 0.079&#xa0;mg/GAE) of SM across the three morphs. The total flavonoid content (TFC) is, however, maximum in leaves followed by seeds and then roots.</p>

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Phytochemical Analysis and Quantification of Phenolics and Flavonoids in Different Morphs of Cleome viscosa Using Different Solvents

  • 期刊论文

  • 发表日期：

    2026年3月30日

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  • Sugandhi Gupta,
  • Veenu Kaul

摘要

Cleome viscosa L. belonging to family Cleomaceae exhibits extensive phenotypic variability. Plants in the current study were separated into long (LM), intermediate (IM) and short (SM) morphs on the basis of their growth habit and branching pattern. Leaf, root and seed tissues of the three morphs were subjected to preliminary analysis for the presence of various phytochemicals followed by estimating the total phenol and flavonoid concentrations in them using the spectrophotometer. Colorimetric tests for ten bioactives were performed. Of the 10, only 8 (Glycosides, alkaloids, flavonoids, steroids, tannins, phenols, coumarins and terpenoids) were detected. Saponins and anthocyanides were not found across morph, solvent and tissue types. Larger number of bioactives were found in the ethanol extract of LM morph. TPC was recorded highest in seeds (5.47 ± 0.011 mg/GAE) and least in roots (1.029 ± 0.079 mg/GAE) of SM across the three morphs. The total flavonoid content (TFC) is, however, maximum in leaves followed by seeds and then roots.