First report on novel indole-3-acetic acid producing bacterium Sinobaca qinghaiensis (EU-PRN-05) from mountainous region of Kinnaur and its effect on growth promotion of pea (Pisum sativum L.)
摘要
The concept of sustainable farming is becoming more and more popular in academic circles and among agricultural communities because the use of chemical fertilizers for plant growth promotion (PGP) is one of the variables that harming the environment and ecosystems. The aim of present study was to assess fertilization and PGP inoculant on growth promotion of pea. In the present investigation, forty eight bacterial strains were isolated from the rhizosphere of pea and screened for indole-3-acetic acid (IAA) production. Using the molecular methods of 16S rRNA gene sequencing, the efficient IAA producing bacterium (EU-PRN-05) was identified as Sinobaca qinghaiensis and then evaluated on pea crop in both controlled and natural environments. Sinobaca qinghaiensis produce IAA ranging from 210.7 ± 0.02 μg/mL−1 in the presence of tryptophan and 26.18 ± 0.04 μg/mL−1 without tryptophan. The result revealed that, application of this bacterium in pea crop under in vitro and in vivo conditions significantly enhanced growth parameters and chlorophyll content. It came to light that, Sinobaca qinghaiensis based biofertilizer formulation had greater ability to enhance pea growth. To best of our knowledge, the present study is the first report on IAA producing Sinobaca qinghaiensis (EU-PRN-05) on growth enhancement of pea. The finding concluded that, application of this identified bacterium was more efficient and could be a useful tool for agriculture growing horticultural crops in mountainous regions.