<p>Aphid being agricultural and horticultural pest develops dense colony on the host foliage through progeny proliferation. Thus, knowledge in progeny proliferation pattern is required for probing aphid control. In the present study, we are testing the hypothesis whether progeny proliferation was mediated by biological clock or not. To test the hypothesis, a number of <i>Myzus persicae</i> like 5, 10, 15, and 20 apterous adults were released on <i>Brassica juncea</i> (NRCHB 101) foliage at dawn and total aphid proliferation (both released and new proliferation) was recorded in dusk (6 PM) or dawn (6 AM). Based on aphid proliferation data, the marginal progeny proliferation from a single aphid from all doses was evaluated to juxtapose between ‘the marginal progeny proliferation from a single aphid’ and ‘the circadian rhythms’ (dusk and dawn). From the result it was evidenced that aphid density is the determining factor for the alignment of significant marginal progeny proliferation from a single aphid with the dusk and dawn. The 20 aphids’ dose aligned the significant marginal progeny proliferation from a single aphid with dawn only unlike 15 aphids’ dose which aligned the significant marginal progeny proliferation from a single aphid with dusk only. The significant marginal progeny proliferation from a single aphid from 5 and 10 aphids’ dose aligned with at least one ‘dusk and dawn’. Thus, aphid progeny proliferation was linked to the circadian rhythms which was again dependent on aphid density for alignment with dawn or dusk.</p>