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<p>The research revealed that expression level of Crystalline (Cry) toxins in various Bt cotton cultivars varies across different crop growth stages. Toxins concentrations in flowers and bolls were quantified using Enzyme Linked Immuno Sorbent Assay (ELISA) at an interval of 75, 90, 105, 120 and 135 days after sowing (DAS). The results revealed a decline in toxin levels with plant maturity with maximum concentration between 90 and 105 DAS. <i>Cry 2Ab</i> levels were consistently higher than <i>Cry 1Ac</i>, with highest expression in flowers followed by bolls. Variation in toxin levels across cultivars and plant parts highlighted potential vulnerabilities to pests, particularly <i>Pectinophora gossypiella</i>, a late season pest with concealed feeding habitat. The findings emphasize the need for strategies to sustain adequate toxin levels during late season growth and recommend the plantation of refugia to delay resistance development in pests, ensuring the continued efficacy of Bt cotton.</p>

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Biochemical Evaluation of Cry Toxin Levels in Various Plant Parts Among Various Growth Stages in Bt Cotton Cultivars

  • 期刊论文

  • 发表日期：

    2026年1月5日

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  • Nitika Saini,
  • Vijay Kumar

摘要

The research revealed that expression level of Crystalline (Cry) toxins in various Bt cotton cultivars varies across different crop growth stages. Toxins concentrations in flowers and bolls were quantified using Enzyme Linked Immuno Sorbent Assay (ELISA) at an interval of 75, 90, 105, 120 and 135 days after sowing (DAS). The results revealed a decline in toxin levels with plant maturity with maximum concentration between 90 and 105 DAS. Cry 2Ab levels were consistently higher than Cry 1Ac, with highest expression in flowers followed by bolls. Variation in toxin levels across cultivars and plant parts highlighted potential vulnerabilities to pests, particularly Pectinophora gossypiella, a late season pest with concealed feeding habitat. The findings emphasize the need for strategies to sustain adequate toxin levels during late season growth and recommend the plantation of refugia to delay resistance development in pests, ensuring the continued efficacy of Bt cotton.