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<p><i>Hygrophila auriculata</i> (Schumach.) Heine is a native of the Indian subcontinent and an underappreciated medicinal plant with enormous nutritional and therapeutic potential. This study developed an effective in vitro propagation protocol for this plant. Traditional propagation techniques have serious problems because of low seed viability and germination capacity, which calls for alternate strategies. Various plant growth regulators, such as 6-Benzylaminopurine (BAP), Indole-3-Acetic Acid (IAA), Indole-3-Butyric Acid (IBA), α-Naphthaleneacetic acid (NAA), and kinetin, were added to shoot tip and nodal segment explants grown on Murashige and Skoog (MS) medium in varying concentrations and combinations. 2.0&#xa0;mg L⁻<sup>1</sup> BAP and 0.5&#xa0;mg L⁻<sup>1</sup> NAA produced the highest regeneration efficiency, yielding response rates of 75% and 71.67% for shoot tip and nodal segment explants, respectively. In addition to superior leaf development and shoot length, this treatment generated maximum shoot proliferation (15 shoots for shoot tips and 13.67 shoots for nodal segments). With MS medium containing 1.0&#xa0;mg L⁻<sup>1</sup> IAA, root induction was most successful, producing 15.33 roots from shoot tips and 17 roots from nodal segment explants. By resolving cultivation issues and facilitating the sustainable use of this priceless plant, the protocol created by this study provides a workable solution for the large-scale propagation of <i>H. auriculata</i>.</p>

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Optimization of In Vitro Propagation Protocol for Conservation and Sustainable Production of Hygrophila auriculata (Schumach.) Heine in an Indian Sub-humid Region

  • 期刊论文

  • 发表日期：

    2025年10月9日

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  • Subrata Das,
  • Gopal Shukla,
  • Bidhan Roy,
  • Shubranil Das,
  • K. Abha Manohar,
  • Sumit Chakravarty

摘要

Hygrophila auriculata (Schumach.) Heine is a native of the Indian subcontinent and an underappreciated medicinal plant with enormous nutritional and therapeutic potential. This study developed an effective in vitro propagation protocol for this plant. Traditional propagation techniques have serious problems because of low seed viability and germination capacity, which calls for alternate strategies. Various plant growth regulators, such as 6-Benzylaminopurine (BAP), Indole-3-Acetic Acid (IAA), Indole-3-Butyric Acid (IBA), α-Naphthaleneacetic acid (NAA), and kinetin, were added to shoot tip and nodal segment explants grown on Murashige and Skoog (MS) medium in varying concentrations and combinations. 2.0 mg L⁻1 BAP and 0.5 mg L⁻1 NAA produced the highest regeneration efficiency, yielding response rates of 75% and 71.67% for shoot tip and nodal segment explants, respectively. In addition to superior leaf development and shoot length, this treatment generated maximum shoot proliferation (15 shoots for shoot tips and 13.67 shoots for nodal segments). With MS medium containing 1.0 mg L⁻1 IAA, root induction was most successful, producing 15.33 roots from shoot tips and 17 roots from nodal segment explants. By resolving cultivation issues and facilitating the sustainable use of this priceless plant, the protocol created by this study provides a workable solution for the large-scale propagation of H. auriculata.