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<p>Aphid is featured with sucking type of insect that siphons host phloem sap through its long and slender stylet. Aphid is gained special concern for a major cause for agricultural and horticultural crop loss globally. Within a short period of time, aphid may colonize on whole host surface and evidenced full crop failure if plant protection measure was not initiated at proper time. Aphid interacts exclusively with host vascular sap with the concomitant secretion of watery saliva. Thus, an ‘interactome’ is developed in phloem sap which was evidenced in the vascular sap composition. Currently, no probe effectively detects early vascular sap changes caused by low aphid infestations. Therefore, the present study aimed to develop a highly sensitive and absorbance-based probe to monitor host vascular sap changes from a lower number of aphid infestation at early time point to differentiate between susceptible and resistant host. The present experiment was carried out in <i>Brassica juncea</i> considering one resistant, RW 85-59 and one susceptible, RH-30 host selected from a panel of 86 accessions through screening with 10 number of <i>Lipaphis erysimi</i> proliferation. A highly sensitive probes, the ratio of 260:215, 260:220 and 280:225 were identified for the vascular sap composition changes from 10 number of <i>Lipaphis erysimi</i> infestation at early time point even at 24&#xa0;h to differentiate between susceptible and resistant hosts even when aphid proliferation was not evidenced as significant difference between two hosts, RW 85-59 and RH-30. The susceptible genotype, RH-30 attenuated any kind of response accumulation in the vascular sap against aphid infestation as compared to the resistant host. Thus, the absorbance ratio of 260:215, 260:220 and 280:225 were identified as a sensitive, absorbance-based non-destructive and novel probe to differentiate resistant and susceptible <i>Brassica juncea</i> accessions at early time point.</p>

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A Novel Absorbance-Based Probe for Early Detection of Aphid-Induced Changes in Vascular Sap To Differentiate Resistant and Susceptible Hosts

  • 期刊论文

  • 发表日期：

    2025年9月20日

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  • Ahanthem Malini Devi,
  • Farzana Ahmad,
  • Balakyntiewshisha Lyngdoh Kynshi,
  • V. Ranjithkumar,
  • Hossain Ali Mondal

摘要

Aphid is featured with sucking type of insect that siphons host phloem sap through its long and slender stylet. Aphid is gained special concern for a major cause for agricultural and horticultural crop loss globally. Within a short period of time, aphid may colonize on whole host surface and evidenced full crop failure if plant protection measure was not initiated at proper time. Aphid interacts exclusively with host vascular sap with the concomitant secretion of watery saliva. Thus, an ‘interactome’ is developed in phloem sap which was evidenced in the vascular sap composition. Currently, no probe effectively detects early vascular sap changes caused by low aphid infestations. Therefore, the present study aimed to develop a highly sensitive and absorbance-based probe to monitor host vascular sap changes from a lower number of aphid infestation at early time point to differentiate between susceptible and resistant host. The present experiment was carried out in Brassica juncea considering one resistant, RW 85-59 and one susceptible, RH-30 host selected from a panel of 86 accessions through screening with 10 number of Lipaphis erysimi proliferation. A highly sensitive probes, the ratio of 260:215, 260:220 and 280:225 were identified for the vascular sap composition changes from 10 number of Lipaphis erysimi infestation at early time point even at 24 h to differentiate between susceptible and resistant hosts even when aphid proliferation was not evidenced as significant difference between two hosts, RW 85-59 and RH-30. The susceptible genotype, RH-30 attenuated any kind of response accumulation in the vascular sap against aphid infestation as compared to the resistant host. Thus, the absorbance ratio of 260:215, 260:220 and 280:225 were identified as a sensitive, absorbance-based non-destructive and novel probe to differentiate resistant and susceptible Brassica juncea accessions at early time point.