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<p>A long and slender stylet is evolved in sucking groups of insects for an efficient tapping of phloem sap from host plants. Sucking groups of insects like aphid, mites cause a huge agricultural and horticultural yield loss globally. It is a long-standing debate whether aphid stylet penetration causes the stylet mediated injury to the host tissue or not. The aphid stylet is penetrated host tissue through rupturing mesophyll cells and reaches to phloem sap which is enriched with sucrose. During navigation of aphid stylet into phloem sieve element cell, numerous mesophyll cells are being ruptured. Till now, it is in the elusive zone that whether aphid stylet penetration induced host response or not against the stylet mediated injury. To configure the issue, the construct having bacterial <i>UidA</i> gene under the promoter of <i>actin depolymerising factor 3</i> (<i>AtADF3</i>; At5g59880) was considered for raising transgenic of the model plant, <i>Arabidopsis thaliana</i>. The <i>ADF3</i> promoter (pADF3) is a constitutive promoter which distinctly evidenced a physical injury mediated host response in the form of <i>GUS</i> staining at the site of physical injury without any changes in <i>UidA</i>,<i> adf3 and tubulin</i> RNA level. Thus, the hypothesis was whether aphid stylet penetration showed <i>GUS</i> staining from stylet mediated injury under <i>ADF3</i> promoter or not. The result indicated that aphid stylet penetration mediated injury (mesophyll cell rupture) in host mesophyll did not induce <i>GUS</i> staining driven by <i>ADF3</i> promoter at the stylet penetration site in <i>Arabidopsis thaliana</i> leaf. Therefore, it was proposed that aphid stylet penetration attenuated host response unlike physical injury and aphid may explore another mechanism to avert host response from stylet penetration mediated injury.</p>

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A Histochemical Portfolio of Physical Injury on Host Tissue from Aphid Stylet Penetration

  • 期刊论文

  • 发表日期：

    2025年6月2日

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  • Hossain Ali Mondal

摘要

A long and slender stylet is evolved in sucking groups of insects for an efficient tapping of phloem sap from host plants. Sucking groups of insects like aphid, mites cause a huge agricultural and horticultural yield loss globally. It is a long-standing debate whether aphid stylet penetration causes the stylet mediated injury to the host tissue or not. The aphid stylet is penetrated host tissue through rupturing mesophyll cells and reaches to phloem sap which is enriched with sucrose. During navigation of aphid stylet into phloem sieve element cell, numerous mesophyll cells are being ruptured. Till now, it is in the elusive zone that whether aphid stylet penetration induced host response or not against the stylet mediated injury. To configure the issue, the construct having bacterial UidA gene under the promoter of actin depolymerising factor 3 (AtADF3; At5g59880) was considered for raising transgenic of the model plant, Arabidopsis thaliana. The ADF3 promoter (pADF3) is a constitutive promoter which distinctly evidenced a physical injury mediated host response in the form of GUS staining at the site of physical injury without any changes in UidA, adf3 and tubulin RNA level. Thus, the hypothesis was whether aphid stylet penetration showed GUS staining from stylet mediated injury under ADF3 promoter or not. The result indicated that aphid stylet penetration mediated injury (mesophyll cell rupture) in host mesophyll did not induce GUS staining driven by ADF3 promoter at the stylet penetration site in Arabidopsis thaliana leaf. Therefore, it was proposed that aphid stylet penetration attenuated host response unlike physical injury and aphid may explore another mechanism to avert host response from stylet penetration mediated injury.