Development of a Culture-Free Herbal-Aided Method for Rapid Detection of Extended Spectrum β-Lactamase in Piggery Farms and Slaughterhouses
摘要
A culture-free herbal aided assay designed for rapid detection of Extended spectrum β-lactamase producing bacterial isolates in piggery farm and slaughter house waste samples. The assay was validated with 309 piggery farm and slaughterhouse waste samples. The relative accuracy and relative sensitivity of the developed assay was found to be 85.1% and 91.3%, respectively in accordance with the culture based double antibiotic disc diffusion assay as reference method.