Efficient Recovery of Vascular Sap from Aphid Infested Brassica sps. Depends on Parameter Combinations Namely, Number of Considered Leaves and Duration of Exudation

For studying phloem-based plant-aphid interaction biology, the vascular sap is a targeted sample for analysis as an ‘interactome’ is developed due to a mixing of aphid saliva through its long and slender stylet to the sieve element cell sap. Therefore, the objective of the present study aimed to identify the maximum vascular sap recovery from different number of leaf and varied exudation time period. The maximum vascular sap recovery was evidenced from the eight leaves from six hours’ exudation period or alternatively four leaves from twenty-four hours’ exudation period. Therefore, the maximum recovery of exudates was correlated with leaf number and exudation period. But, the ratio of 260 and 280 nm was not changed in all parameter’s combination. This information will act as a functional probe to identify the maximum exudates recovery in a species-specific manner in a short period of time in plant-aphid interaction biology for addressing phloem-based defense mechanism.