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Purpose <p>Hollow manganese dioxide (HMnO<sub>2</sub>) nanoparticles offer significant potential for biomedical and biosensing applications. This study presents a pH- and glutathione (GSH) responsive biodegradable HMnO₂-based drug delivery system designed to enhance chemodynamic therapy through targeted drug release.</p> Methods <p>HMnO<sub>2</sub> nanoparticles were synthesized with high doxorubicin hydrochloride (DOX) loading efficiency. The DOX-loaded HMnO<sub>2</sub> was coated with macrophage cell membranes (MCM) to improve biocompatibility. A MUC1-targeting DNA aptamer was conjugated to develop the Apt-HMnO<sub>2</sub>-DOX-MCM construct. In vitro, assays evaluated DOX release under acidic and elevated GSH conditions and assessed Fenton-like catalytic activity. In vivo, efficacy was tested in B16F0 tumor-bearing C57BL/6 mice. Magnetic resonance imaging (MRI) capabilities were also investigated.</p> Results <p>HMnO<sub>2</sub> nanoparticles demonstrated a high DOX encapsulation efficiency of 94% ± 2.8 and a loading content of 38% ± 1.1. Apt-HMnO<sub>2</sub>-DOX-MCM significantly increased cytotoxicity cellular uptake and inhibited colony formation and migration in MUC1-overexpressing B16F0 cells. In vivo studies showed marked tumor size reduction compared to non-targeted controls. Both Apt-HMnO<sub>2</sub>-DOX-MCM and HMnO<sub>2</sub>-DOX-MCM functioned effectively as T1- and T2-weighted MRI contrast agents.</p> Conclusion <p>The Apt-HMnO<sub>2</sub>-DOX-MCM construct represents a promising biocompatible theranostic platform that combines targeted drug delivery with diagnostic imaging to potentiate chemodynamic cancer therapy.</p>

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Smart dual-responsive theranostic MUC1 aptamer-tagged macrophage cell membrane–coated hollow manganese dioxide for chemodynamic therapy and MR imaging of melanoma

  • 期刊论文

  • 发表日期：

    2025年5月16日

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  • Mina Alikhani,
  • Mahsa Nazari,
  • Sirous Nekooei,
  • Khalil Abnous,
  • Seyed Mohammad Taghdisi,
  • Farrokh Seilanian Toosi,
  • Mohammad Ramezani,
  • Mona Alibolandi

摘要

Purpose

Hollow manganese dioxide (HMnO2) nanoparticles offer significant potential for biomedical and biosensing applications. This study presents a pH- and glutathione (GSH) responsive biodegradable HMnO₂-based drug delivery system designed to enhance chemodynamic therapy through targeted drug release.

Methods

HMnO2 nanoparticles were synthesized with high doxorubicin hydrochloride (DOX) loading efficiency. The DOX-loaded HMnO2 was coated with macrophage cell membranes (MCM) to improve biocompatibility. A MUC1-targeting DNA aptamer was conjugated to develop the Apt-HMnO2-DOX-MCM construct. In vitro, assays evaluated DOX release under acidic and elevated GSH conditions and assessed Fenton-like catalytic activity. In vivo, efficacy was tested in B16F0 tumor-bearing C57BL/6 mice. Magnetic resonance imaging (MRI) capabilities were also investigated.

Results

HMnO2 nanoparticles demonstrated a high DOX encapsulation efficiency of 94% ± 2.8 and a loading content of 38% ± 1.1. Apt-HMnO2-DOX-MCM significantly increased cytotoxicity cellular uptake and inhibited colony formation and migration in MUC1-overexpressing B16F0 cells. In vivo studies showed marked tumor size reduction compared to non-targeted controls. Both Apt-HMnO2-DOX-MCM and HMnO2-DOX-MCM functioned effectively as T1- and T2-weighted MRI contrast agents.

Conclusion

The Apt-HMnO2-DOX-MCM construct represents a promising biocompatible theranostic platform that combines targeted drug delivery with diagnostic imaging to potentiate chemodynamic cancer therapy.